- 著者
- Eiichi TAHARA Tadamichi MITSUMA Yutaka SHIMADA Takashi ITOH Katsutoshi TERASAWA
- 出版者
- The Japan Society for Oriental Medicine
- 雑誌
- Kampo Medicine (ISSN:02874857)
- 巻号頁・発行日
- vol.48, no.3, pp.341-348, 1997-11-20 (Released:2010-03-12)
- 参考文献数
- 17
- 被引用文献数
- 1 2
今回我々はバセドウ病による甲状腺機能亢進症に対して抗甲状腺剤を用いることなく, 漢方方剤のみによる治療を行い, 寛解に至った症例を報告した。症例1は47歳, 女性。約2年前より動悸, 体重減少, イライラ感, 耳鳴, めまい感など多愁訴。TSH低値, fT3・fT4・TBII高値, 123I 24時間摂取率高値からバセドウ病と診断。動悸などを目標に炙甘草湯を処方。その後証に応じて柴胡加龍骨牡蛎湯などを併用した。1年10ヶ月後には, TSH, fT3, fT4, TBIIとも正常化し, 以後現在まで正常域。症例2は40歳, 女性。3年前より動悸出現。近医で甲状腺機能亢進症を指摘され, 約2ヶ月間チアマゾールを内服。初診時TSH低値, fT3・fT4正常, TBII高値。動悸などを目標に炙甘草湯を処方。約3週後にチアマゾールを自己中止。証に応じて柴胡加龍骨牡蛎湯などを併用した。fT3, fT4は徐々に増加したが動悸は消失。10ヶ月後からfT3・fT4は徐々に低下傾向にある。甲状腺機能亢進症に対して抗甲状腺剤を用いることなく, 漢方治療のみで寛解に至らしめ得る病態のあることが示唆された。
- 著者
- Keito Mineta Junya Hirota Kesuke Yamada Takashi Itoh Poyu Chen Hidekazu Iwakawa Hirotomo Takatsuka Yuji Nomoto Masaki Ito
- 出版者
- Japanese Society for Plant Biotechnology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.40, no.4, pp.353-359, 2023-12-25 (Released:2023-12-25)
- 参考文献数
- 20
Although it is well known that hierarchical transcriptional networks are essential for various aspects of plant development and environmental response, little has been investigated about whether and how they also regulate the plant cell cycle. Recent studies on cell cycle regulation in Arabidopsis thaliana identified SCARECROW-LIKE28 (SCL28), a GRAS-type transcription factor, that constitutes a hierarchical transcriptional pathway comprised of MYB3R, SCL28 and SIAMESE-RELATED (SMR). In this pathway, MYB3R family proteins regulate the G2/M-specific transcription of the SCL28 gene, of which products, in turn, positively regulate the transcription of SMR genes encoding a group of plant-specific inhibitor proteins of cyclin-dependent kinases. However, this pathway with a role in cell cycle inhibition is solely demonstrated in A. thaliana, thus leaving open the question of whether and to what extent this pathway is evolutionarily conserved in plants. In this study, we conducted differential display RT-PCR on synchronized Nicotiana tabacum (tobacco) BY-2 cells and identified several M-phase-specific cDNA clones, one of which turned out to be a tobacco ortholog of SCL28 and was designated NtSCL28. We showed that NtSCL28 is expressed specifically during G2/M and early G1 in the synchronized cultures of BY-2 cells. NtSCL28 contains MYB3R-binding promoter elements, so-called mitosis-specific activator elements, and is upregulated by a hyperactive form of NtmybA2, one of the MYB3R proteins from tobacco. Our study indicated that a part of the hierarchical pathway identified in A. thaliana is equally operating in tobacco cells, suggesting the conservation of this pathway across different families in evolution of angiosperm.
- 著者
- Akihiko Yamagishi Shin-ichi Yokobori Yoshitaka Yoshimura Masamichi Yamashita Hirofumi Hashimoto Takashi Kubota Hajime Yano Junichi Haruyama Makoto Tabata Kensei Kobayashi Hajime Honda Yuichi Utsumi Tsunemasa Saiki Takashi Itoh Atsuo Miyakawa Kenji Hamase Takeshi Naganuma Hajime Mita Kenichi Tonokura Sho Sasaki Hideaki Miyamoto
- 出版者
- 日本宇宙生物科学会
- 雑誌
- Biological Sciences in Space (ISSN:09149201)
- 巻号頁・発行日
- vol.24, no.2, pp.67-82, 2010 (Released:2012-06-26)
- 参考文献数
- 114
- 被引用文献数
- 1 12
Liquid water is considered to be critical for life. Gibbs free energy is another factor that is important to sustain life for long durations. Gibbs free energy is obtained by reactions between reductants and oxidants, or from any other non-equilibrium state of matter. As an example, aerobic organisms use carbohydrates and oxygen to obtain energy. Many types of chemoautotrophic mechanisms are known for this process as well. On the surface of Mars, methane and oxidative compounds such as ferric oxide, sulfate and perchloride, which could provide redox-derived Gibbs free energy, have been detected. Iron-dependent methane oxidizing bacteria have been found in marine environments on Earth. This finding suggests the possible presence of methane-oxidizing bacteria on the Mars surface, if the local thermal environment and other resources permit proliferation and metabolism of bacteria. Our project aims to search for methane-oxidizing microbes on the Mars surface. Martian soil will be sampled from a depth of about 5 - 10 cm below the surface, where organisms are expected to be protected from the harsh hyper-oxidative environment of the Mars surface. Small particles less than 0.1 mm or 1 mm will be sieved from the sample, before being transferred to the analysis section by a micro-actuator. The particles will be stained with a cocktail of fluorescent reagents, and examined by fluorescence microscopy. A combination of fluorescent dyes has been selected to identify life forms in samples. A membrane-specific dye or a combination of dyes will be used to detect membranes surrounding the "cell". An intercalating fluorescent dye such as SYBR Green will be used to detect genetic compounds such as DNA. A substrate dye that emits fluorescence upon cleavage by a catalytic reaction will be used to detect the catalytic activity of the "cell". A combination of staining reagents has been chosen based on the definition of life. A membrane separating a cell from the ambient environment may lead to identification of an "individual". DNA or genetic material is required for "replication" of the life form. Catalytic reactions carried out by enzymes drive "metabolism". This combination of strategies will also be useful for detecting pre-biotic organic material as well as remnants of ancient life. Hydrolysis of the polymers in the "cell" followed by HPLC or soft ionization MS for amino acid analysis will be effective for examining whether Martian life is identical to or different from terrestrial life. The number and type of the amino acids as well as their chirality will be analyzed to distinguish whether the polymers are contaminants from Earth.