著者

Ying An Tetsuro Yamashita Atsushi Seino Kunio Imai Koichi Suzuki

出版者

The Japanese Society of Sericultural Science

雑誌

Journal of Insect Biotechnology and Sericology (ISSN:13468073)

巻号頁・発行日

vol.76, no.1, pp.1_51-1_55, 2007 (Released:2008-10-06)

参考文献数

27

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Peptides of the ENF (Glu-Asn-Phe) family, which occur in the hemolymph of Lepidoptera, were reported to act on the plasmatocytes and heart, cause body paralysis, and inhibit body growth. In this paper we report on yet another, most surprising effect. An ENF peptide isolated from the wild silkmoth, Antheraea yamamai, and named Antya-ParP causes paralysis in the caterpillars but not in the pupae of Bombyx mori. However, adults that emerge from the treated pupae lay diapausing eggs. The embryonic diapause of B. mori is normally induced by the diapause hormone (Bommo-DH), which is produced in pupae from the suboesophageal ganglion. The effect of Antya-ParP is independent of this ganglion, showing that the Antya-ParP action is not mediated by Bommo-DH. The sequence of 23 amino acid residues of Antya-ParP shows no similarity to the Bommo-DH sequence of 24 residues. The mode of action of the two peptides seems to be identical but the threshold concentration of Bommo-DH is up to hundred times lower than that of Antya-ParP.

著者

Masaaki KATAYAMA Shinobu TSUCHIAKA Tomoki MOTEGI Masao MIYAZAKI Tetsuro YAMASHITA Shunsuke SHIMAMURA Yasuhiko OKAMURA Yuji UZUKA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0565, (Released:2014-03-05)

被引用文献数

2

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Phosphate-buffered sucrose (PBSc) solution is effective for short-term hypothermic preservation of tissue during feline kidney transplantation. A high-molecular-weight polyethylene glycol (35,000 Da, PEG35) reportedly enhanced the protective effects against cold-induced tubular injuries in animal kidney transplantation models. We investigated the ability of PBSc solution containing PEG35 to preserve cultured feline kidney cells using in vitro WST-8 cell proliferation assays. PEG35 significantly improved cell viability during 24 hr of cold preservation. PBSc containing 20 g/l PEG35 achieved an effect almost equal to that of University of Wisconsin (UW) solution, the gold standard preservation solution used in human clinical kidney transplantation, for up to 24 hr of preservation. Our results suggest that PBSc containing PEG35 provides an excellent medium for graft cold storage during feline kidney transplantation.