- 著者
- Eri Kikkawa Masafumi Tanaka Tomi T. Tsuda Koichi Murata Taeko K. Naruse Akinori Kimura
- 出版者
- Japanese Society for Histocompatibility and Immunogenetics
- 雑誌
- 日本組織適合性学会誌 (ISSN:21869995)
- 巻号頁・発行日
- vol.26, no.3, pp.195-203, 2019 (Released:2019-12-27)
- 参考文献数
- 34
Endangered penguins are in need of the genetic management to estimate the risk for loss of genetic diversity from populations resulting from habitat fragmentation or inbreeding. However, as for genome diversity in penguin species, there are limited reports and it is insufficient to give useful and appropriate information. In the present study, we obtained the full length sequence of the MHC class II gene, which is expected to be a useful genetic marker for biodiversity in conservation genetics. The 4.4 kb genome region containing two novel genes was determined for nucleotide sequences using genomic DNA extracted from Humboldt penguin, by using the inverse PCR method. Homology analysis of MHC class II genes with those from other birds suggested that the novel two genes were alpha and beta genes. In addition, phylogenetic analysis suggested that the beta gene of penguins was clustered with the beta genes from waterfowl. These observations provide basic information on the structure of MHC class II locus to relieve the genetic diversity of penguin species.
- 著者
- Masamichi KUWAJIMA Hiroaki FUJIHARA Hiroyoshi SEI Asako UMEHARA Masako SEI Tomi T. TSUDA Akiko SUKENO Tatsuya OKAMOTO Akiko INUBUSHI Yoichi UETA Toshio DOI Hiroshi KIDO
- 出版者
- The Japan Endocrine Society
- 雑誌
- Endocrine Journal (ISSN:09188959)
- 巻号頁・発行日
- vol.54, no.6, pp.911-925, 2007 (Released:2008-02-20)
- 参考文献数
- 46
- 被引用文献数
- 13 14
The mechanism of onset of hypoglycemia in patients with carnitine deficiency has yet to be determined. Using mice with systemic carnitine deficiency (JVS mice), we examined this mechanism, focusing on the weaning period (days 14-28 postpartum). For normal mice, the survival rate was 100%, and no hypoglycemia was observed at all. Gastric lactose began to decrease on day 17, and cellulose increased sharply in amount thereafter. For JVS mice, the survival rate was 77% on day 14 and 28% on day 28. From day 21 on, hypoglycemia was noted. Gastric lactose had disappeared almost completely by day 17, and cellulose was almost undetectable from days 14 to 28. Expression of orexin mRNA in the hypothalamus did not differ between JVS and normal mice on day 14, but was suppressed in JVS mice on days 21 and 28. When JVS mice were fed a carnitine-rich diet, suppression of expression of orexin mRNA in hypothalamus was eliminated, and on day 28 lactose and cellulose were detected in the stomach without hypoglycemia. In conclusion, the suppression of the expression of orexin in the hypothalamus during the weaning period may be involved in the marked anorexia in JVS mice, which eventually leads to death from hypoglycemia.