- 著者
- Kei YOSHIDA Toshio MATSUOKA Yui KOBATAKE Satoshi TAKASHIMA Naohito NISHII
- 出版者
- JAPANESE SOCIETY OF VETERINARY SCIENCE
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- pp.21-0325, (Released:2022-01-03)
- 被引用文献数
- 2
The present study aimed to quantitatively evaluate muscle mass and gene expression in dogs with glucocorticoid-induced muscle atrophy. Five healthy beagles received oral prednisolone for 4 weeks (1 mg/kg/day), and muscle mass was then evaluated via computed tomography. Histological and gene expression analyses were performed using biopsy samples from the biceps femoris before and after prednisolone administration. The cross-sectional area of the third lumbar paraspinal and mid-femoral muscles significantly decreased after glucocorticoid administration (from 27.5 ± 1.9 to 22.6 ± 2.0 cm2 and from 55.1 ± 4.7 to 50.7 ± 4.1 cm2, respectively; P<0.01). The fast- and slow-twitch muscle fibers were both atrophied (from 2,779 ± 369 to 1,581 ± 207 μm2 and from 2,871 ± 211 to 1,971 ± 169 μm2, respectively; P<0.05). The expression of the growth factor receptor-bound protein 10 (GRB10) significantly increased after prednisolone administration (P<0.05). Because GRB10 suppresses insulin signaling and the subsequent mammalian target of rapamycin complex 1 activity, increased expression of GRB10 may have resulted in a decrease in protein anabolism. Taken together, 1 mg/kg/day oral prednisolone for 4 weeks induced significant muscle atrophy in dogs, and GRB10 might participate in the pathology of glucocorticoid-induced muscle atrophy in canines.
- 著者
- Tsuneo Watanabe Nobuo Terabayashi Bateer Shi Sohee Shin Kosho Kasuga Tamotsu Yabumoto Katsuji Shimizu Toshio Matsuoka
- 出版者
- 一般社団法人日本体力医学会
- 雑誌
- The Journal of Physical Fitness and Sports Medicine (ISSN:21868131)
- 巻号頁・発行日
- vol.2, no.2, pp.243-250, 2013-05-25 (Released:2013-06-21)
- 参考文献数
- 24
- 被引用文献数
- 1 3
The purpose of this study was to investigate the influence of cryotherapy on knee joint position sense (JPS) and intraarticular blood flow volume (IBFV) and evaluate their relationships with cooling time as well as with surface temperature and deep temperature. Ten healthy volunteers were examined. This study consisted of a same-subjects repeated-measures design, with the timeframe of cryotherapy application (no therapy [resting control group], 2-min cooling, or 15-min cooling intervention) after exercise being the independent variable, and IBFV, knee JPS, surface temperature, and deep temperature serving as the dependent variables. Dependent variables were examined before 10-min cycle ergometer exercise (baseline), post-exercise, post-cooling, and 15 min later. In the 15-min cooling group, IBFV immediately after cooling and 15 min later were significantly lower than the post-exercise values (P = 0.048 and 0.016, respectively), and knee JPS at 15 min later was significantly lower than the baseline value (P = 0.037). By contrast, the 2-min cooling group showed no significant changes in either knee JPS or IBFV. Although both surface and deep temperatures after cooling were significantly lower than baseline (P = 0.034 and P < 0.001, respectively) in the 2-min cooling group, 15 min later they were significantly higher than post-cooling values (P = 0.023 and 0.023, respectively). These results suggest that 15-min cooling interventions functionally impair the sensitivity of JPS, although cooling is suitable for reduction of IBFV in deep tissue. Cooling interventions lasting less than 2-min did not affect knee JPS; however no reduction of IBFV occurred during this timeframe.