- 著者
- Chao ZHAO Kun ZHAO Xiaoyan LIU Yi-fan HUANG Bin LIU
- 出版者
- Japanese Society for Food Science and Technology
- 雑誌
- Food Science and Technology Research (ISSN:13446606)
- 巻号頁・発行日
- vol.19, no.4, pp.661-667, 2013 (Released:2013-09-05)
- 参考文献数
- 35
- 被引用文献数
- 6 10
Orthogonal experiment was used to optimize the extraction conditions of Flammulina velutipes mycelia polysaccharides (FvP). Four independent variables (ratio of water to raw material, initial pH value, extracting temperature, and extracting time) were taken into consideration. A yield of FvP of 2.19% was obtained under an optimized condition (ratio of water to material of 50:1, initial pH value of 6.0, extracting temperature of 85°C, and extracting time of 6 h). Subsequently, antioxidative properties of FvP-2 (crude polysaccharides) and FvP-3 (deproteinized polysaccharides) prepared from F. velutipes mycelia were evaluated by monitoring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical scavenging activity, together with antitumor activity against the human hepatocellular carcinoma BEL-7402 cells. DPPH scavenging rate of 65.85% and hydroxyl radical scavenging rate of 71.24% were obtained at 200 μg/mL of FvP-3. The inhibition rate of BEL-7402 cells was up to 45% at 640 μg/mL of FvP-2. These results suggested that FvP possesses potent antioxidant and antitumor properties. The polysaccharide may be useful as a functional food additive and an antioxidant and antitumor agent.
- 著者
- Xiaoyan Liu Prakash Babu Adhikari Ryushiro D. Kasahara
- 出版者
- Japan Mendel Society, International Society of Cytology
- 雑誌
- CYTOLOGIA (ISSN:00114545)
- 巻号頁・発行日
- vol.88, no.1, pp.35-39, 2023-03-25 (Released:2023-03-25)
- 参考文献数
- 16
Sexual reproduction in angiosperms is a complex and precise process of regulation, which includes the pollen tube guidance, double fertilization, and the seed development process. The previous report identified that for seed development, there is a period of preparation for fertilization (between pollen tube guidance and double fertilization) that is called pollen-tube-dependent ovule enlargement morphology (POEM). It has been shown that the pollen tube content (PTC) plays a crucial role in the enlargement of ovules and the initiation of seed coat formation. However, we did not investigate the potential of endosperm proliferation in autonomous mutants at a later stage. Here, we investigated this phenomenon using vanillin staining and transparent experiments to examine the manner, in which the PTC affects the potential of endosperm formation. Interestingly, the PTC increased the number of endosperm nuclei without fertilization equally and synchronously in mea and fis2 ovules. This finding might help improve the study of apomixes and our understanding of how the molecular mechanisms that regulate this phenomenon will contribute to plant reproductive science in the future.