著者

張 敦福 魏 泉 李 軒羽 Dunfu Zhang Quan Wei Xuanyu Li

雑誌

関西学院大学社会学部紀要 (ISSN:04529456)

巻号頁・発行日

no.134, pp.27-38, 2020-03-12

著者

李 軒羽 Xuanyu Li

雑誌

関西学院大学先端社会研究所紀要 = Annual review of the institute for advanced social research (ISSN:18837042)

巻号頁・発行日

no.19, pp.83-94, 2022-03-31

著者

Mingqun Li Yuqin Huang Hongli Xi Wei Zhang Ziwu Xiang Lingyun Wang Xuanyu Li Hongyan Guo

出版者

The Japan Endocrine Society

雑誌

Endocrine Journal (ISSN:09188959)

巻号頁・発行日

vol.69, no.9, pp.1067-1078, 2022 (Released:2022-09-28)

参考文献数

30

被引用文献数

4

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Gestational diabetes mellitus (GDM) is a health risk for pregnant women and infants. Emerging evidence suggests that the deregulation of circular RNAs (circRNAs) is associated with the progression of this disorder. The objective of this study was to investigate the role of circ_FOXP1 in GDM. Cell models of GDM were established by treating human trophoblast cells with high glucose (HG). The expression of circ_FOXP1, miR-508-3p and SMAD family member 2 (SMAD2) mRNA was detected by quantitative real-time PCR (qPCR). Cell proliferation was assessed by EdU assay and MTT assay, and cell cycle and cell apoptosis were determined by flow cytometry assay. The protein levels of proliferation- and apoptosis-related markers and SMAD2 were measured by western blot. The relationship between miR-508-3p and circ_FOXP1 or SMAD2 was validated by dual-luciferase reporter assay or pull-down assay. The expression of circ_FOXP1 was downregulated in HG-treated HTR-8/SVneo cells. Circ_FOXP1 overexpression promoted HG-inhibited HTR-8/SVneo cell proliferation and suppressed HG-induced HTR-8/SVneo cell cycle arrest and apoptosis. Circ_FOXP1 positively regulated the expression of SMAD2 by targeting miR-508-3p. MiR-508-3p was overexpressed in HG-treated HTR-8/SVneo cells, and its overexpression reversed the effects of circ_FOXP1 overexpression. MiR-508-3p inhibition also alleviated HG-induced HTR-8/SVneo cell injuries, while the knockdown of SMAD2 abolished these effects. Collectively, circ_FOXP1 promotes the growth and survival of HG-treated human trophoblast cells through the miR-508-3p/SMAD2 pathway, hinting that circ_FOXP1 was involved in GDM progression.

著者

Xuanyu Li Xuelong Jin

出版者

Japan Brain Science society

雑誌

脳科学誌 (ISSN:13415301)

巻号頁・発行日

vol.38, pp.21-34, 2012-03-30 (Released:2017-06-01)

参考文献数

29

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Central nervous system (CNS) injuries lead to severe and permanent neurological deficits. Fortunately, as a result of the rapid progress in biomedical science, particularly the stem cell biology, stem cell transplantation holds promise for promoting anatomical repair and functional recovery after traumatic or ischemic injuries to the CNS. However, the tumorigenic potential of these cells remains a great concern, as reflected in the formation of teratomas by transplanted pluripotent cells. This gap in the field is particularly serious as stem cell tumori-genicity represents the key obstacle to the safe use of stem cell-based therapy for CNS injuries. Although some adult stem cell therapies appear to be safe, they have only a very narrow range of uses in human disease. In this review, the links between pluripotency and tumorigenicity are explored. And new medical imaging modalities for more accurately testing the tumorigenic potential of iPSC and of other stem cells applicable to regenerative medicine are proposed. Finally, the most promising emerging approaches for overcoming the challenges of stem cell tumorigenicity are highlighted.