著者
Suranjith Bandara KORALEGEDARA Chuan-Yao LIN Yang-Fan SHENG
出版者
Meteorological Society of Japan
雑誌
気象集誌. 第2輯 (ISSN:00261165)
巻号頁・発行日
vol.97, no.4, pp.821-839, 2019 (Released:2019-08-02)
参考文献数
52
被引用文献数
3

In this present study, we analyzed the synoptic and mesoscale dynamics and underlying mechanism of an extreme rainfall and flood event that occurred in Sri Lanka between 14-17 May 2016, using the Weather Research and Forecasting Model simulations with a horizontal grid size of 3 km and observational data. This extreme rainfall event was associated with a low-pressure system (LPS) that originated over the Bay of Bengal in the Indian Ocean and passed over Sri Lanka. The observed maximum accumulation of rainfall during the event exceeded 300 mm at several weather stations on 15-16 May and it resulted in severe flooding and landslides, particularly in the western part of the island. The model closely simulated the timing of the initiation of the LPS and its development along the east coast of Sri Lanka. The model could capture the overall rainfall tendency and pattern of this event. Synoptic and mesoscale analyses indicated that this extreme rainfall event occurred as the cumulative effect of a sustained low-level convergence zone, generated by an enhanced westerly monsoon flow and the circulation of the LPS, alongside a continuous supply of high-magnitude moisture, strong vertical motion, and orographic effects of the Central Mountains of Sri Lanka. Model sensitivity experiments indicated that the rainfall over the western slope area of the mountains was enhanced by mountain lifting, whereas western coastal rainfall was reduced because the mountains blocked the northeasterly flow of the LPS.
著者
CAO Lili CHENG Ronghua YAO Lin YUAN Shuxian YAO Xinhua
出版者
公益社団法人 日本獣医学会
雑誌
The Journal of Veterinary Medical Science (ISSN:09167250)
巻号頁・発行日
vol.76, no.1, pp.9-14, 2014
被引用文献数
10

The Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high simply, specificity, sensitivity and rapidity. In this study, A LAMP assay with 6 primers targeting a highly conserved region of the GRA1 gene was developed to diagnose <i>Toxoplasma gondii</i>. The reaction time of the LAMP assay was shortened to 30 min after optimizing the reaction system. The LAMP assay was found to be highly specific and stable. The detection limit of the LAMP assay was 10 copies, the same as that of the conventional PCR. We used the LAMP assay to develop a real-time fluorogenic protocol to quantitate <i>T. gondii</i> DNA and generated a log-linear regression plot by plotting the time-to-threshold values against genomic equivalent copies. Furthermore, the LAMP assay was applied to detect <i>T. gondii</i> DNA in 423 blood samples and 380 lymph node samples from 10 pig farms, and positive results were obtained for 7.8% and 8.2% of samples, respectively. The results showed that the LAMP method is slightly more sensitive than conventional PCR (6.1% and 7.6%). Positive samples obtained from 6 pig farms. The LAMP assay established in this study resulted in simple, specific, sensitive and rapid detection of <i>T. gondii</i> DNA and is expected to play an important role in clinical detection of <i>T. gondii</i>.