著者

Katsuhito TAKEI Nobuyuki SERIZAWA Tokujiro NISHIKIORI Yasuhiko ITO

出版者

The Electrochemical Society of Japan

雑誌

Electrochemistry (ISSN:13443542)

巻号頁・発行日

vol.86, no.2, pp.35-37, 2018-03-05 (Released:2018-03-05)

参考文献数

12

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The cathodic reduction of nitrogen (N2) gas in molten chloride was investigated using a porous nickel (Ni) electrode. A LiCl-KCl-CsCl melt containing cathodically formed nitride ion (N3−) was sampled from an electrolytic bath and analyzed quantitatively by ion chromatography. The current efficiency of the cathodic reaction calculated from the ratio of quantity of the produced N3− was approximately 78% on average. The main reason why the measured efficiency did not reach 100% is considered as the amount of N3− increased more in the pores of the gas electrode than in the bulk of the electrolytic bath.

著者

Sangchai Yingsakmongkon Daisei Miyamoto Nongluk Sriwilaijaroen Kimie Fujita Kosai Matsumoto Wipawee Jampangern Hiroaki Hiramatsu Chao-Tan Guo Toshihiko Sawada Tadanobu Takahashi Kazuya Hidari Takashi Suzuki Morihiro Ito Yasuhiko Ito Yasuo Suzuki

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.31, no.3, pp.511-515, 2008-03-01 (Released:2008-03-01)

参考文献数

30

被引用文献数

42 46

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Using a plaque reduction assay, treatment of human influenza A viruses with the fruit-juice concentrate of Japanese plum (Prunus mume SIEB. et ZUCC) showed strong in vitro anti-influenza activity against human influenza A viruses before viral adsorption, but not after viral adsorption, with 50% inhibitory concentration (IC50) values against A/PR/8/34 (H1N1) virus, A/Aichi/2/68 (H3N2) virus and A/Memphis/1/71 (H3N2) virus of 6.35±0.17, 2.84±1.98 and 0.53±0.10 μg/ml, respectively. The plum-juice concentrate exhibited hemagglutination activity toward guinea pig erythrocytes. Its hemagglutination activity was inhibited by the monosaccharide N-acetylneuraminic acid and a sialoglycoprotein (fetuin), but not by the other tested monosaccharides (mannose, galactose, glucose and N-acetylglucosamine), suggesting the presence of a lectin-like molecule(s) in the Japanese plum-juice concentrate. Our findings suggest that the fruit-juice concentrate of Japanese plum may prevent and reduce infection with human influenza A virus, possibly via inhibition of viral hemagglutinin attachment to host cell surfaces by its lectin-like activity.

著者

Hikaru TAKESHITA Eri WATANABE Yoshihiko NOROSE Yasuhiko ITO Hidemi TAKAHASHI

出版者

Biomedical Research Press

雑誌

Biomedical Research (ISSN:03886107)

巻号頁・発行日

vol.40, no.2, pp.87-95, 2019-04-01 (Released:2019-04-13)

参考文献数

22

被引用文献数

2

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Helicobacter pylori (H. pylori) urease is a key protein for persistent infection of the bacteria in the stomach. Although H. pylori generally induce anti-H. pylori-specific antibodies (Abs), these Abs do not usually work for eradication or prevention of the H. pylori infection. In our previous study, we identified a linear epitope composed of 19-mer peptides termed UB-33, CHHLDKSIKEDVQFADSRI, within the large subunit of H. pylori urease. Anti-UB-33-specific Abs neutralized the enzymatic activity of H. pylori urease in vitro. In the present study, we evaluated the effect of immunization of BALB/c mice with H. pylori UB-33 peptide. After confirming the production of anti-UB-33-specific Abs, mice were challenged orally with H. pylori Sydney Strain-1 (SS-1). Mice producing anti-UB-33-specific Abs were not infected with SS-1, and the amount of SS-1 isolate in their stomach was significantly reduced. Also, the urease-negative mutant of H. pylori, HPP1801, did not colonize in the stomach, indicating that H. pylori urease was a critical element for infection of H. pylori in the gastric mucosa. Moreover, mice producing UB-33-specific Abs apparently suppressed H. pylori infection in the stomach where anti-UB-33 Abs were secreted in the gastric juice, indicating that H. pylori colonization was inhibited in the presence of anti-UB-33 Abs. In addition, the neutralization activity of sera from mice immunized with purified urease was less potent than that in the sera from mice immunized with UB-33. Furthermore, the recognition of epitope UB-33 was mediated through Toll-like receptor 2 (TLR2) on the B-1 cells using TLR2-knockout BALB/c mice in vivo. These results indicate that liner peptide UB-33 should be used for immunization to induce neutralizing Abs instead of purified H. pylori urease to prevent H. pylori infection and their colonization in the stomach.