著者

中田 善啓 Yoshihiro Nakata

出版者

甲南大学経営学会

雑誌

甲南経営研究 = Konan business review (ISSN:04524152)

巻号頁・発行日

vol.58, no.3, pp.51-86, 2017-12

著者

Hiroki Ikeda Tomoki Shibuya Manabu Nishiyama Yoshihiro Nakata Yoshinori Kanayama

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-015, (Released:2016-09-28)

被引用文献数

21

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Calcium (Ca2+) concentration, early fruit growth, and expression of Ca2+-movement-related genes were analyzed during early fruit development in the tomato, which is the most important stage regarding the incidence of blossom-end rot (BER), to investigate the physiological mechanisms affecting the occurrence of BER. We used tomato introgression line IL8-3 with a chromosome segment from a wild relative (Solanum pennellii) because this line shows lower incidence of BER compared with the parent cultivar ‘M82’ (S. lycopersicum), as described previously. Ca2+ concentration in fruit and leaves was higher in IL8-3 than in ‘M82’, whereas no significant differences were observed between Ca2+ concentration in roots and stems of ‘M82’ and IL8-3. These results suggested that a Ca2+ transport property is an essential factor for the lower incidence of BER in IL8-3. IL8-3 fruit showed a lower growth rate than ‘M82’, which could result in preventing the occurrence of BER. The expression of genes encoding cation exchangers, Ca2+-ATPases, a Ca2+ channel, and Na+/Ca2+ exchangers, was higher in IL8-3 fruit than in ‘M82’ fruit, suggesting active Ca2+ movement in IL8-3. All results in this study could be related to physiological mechanisms accounting for the lower incidence of BER in IL8-3.

著者

Kanako Miyano Yuka Sudo Akinobu Yokoyama Kazue Hisaoka-Nakashima Norimitsu Morioka Minoru Takebayashi Yoshihiro Nakata Yoshikazu Higami Yasuhito Uezono

出版者

(公社)日本薬理学会

雑誌

Journal of Pharmacological Sciences (ISSN:13478613)

巻号頁・発行日

pp.14R13CP, (Released:2014-11-22)

参考文献数

30

被引用文献数

7 45

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The G protein–coupled receptors (GPCRs) form the largest and the most versatile superfamily that share a seven-transmembrane-spanning architecture. GPCR-signaling is involved in vision, taste, olfaction, sympathetic/parasympathetic nervous functions, metabolism, and immune regulation, indicating that GPCRs are extremely important therapeutic targets for various diseases. Cellular dielectric spectroscopy (CDS) is a novel technology that employs a label-free, real-time and cell-based assay approach for the comprehensive pharmacological evaluation of cells that exogenously or endogenously express GPCRs. Among the biosensors that use CDS technology, the CellKeyTM system not only detects the activation of GPCRs but also distinguishes between signals through different subtypes of the Gα protein (Gs, Gi/o, and Gq). In this review, we discuss the traditional assays and then introduce the principles by which the CellKeyTM system evaluates GPCR activation, followed by a perspective on the advantages and future prospects of this system.