- 著者
- Zong Min Satoh Ayano Yu Mei Kuen Siu Ka Yu Ng Wing Yan Chan Hsiao Chang Tanner Julian A. Yu Sidney
- 雑誌
- PLoS ONE (ISSN:19326203)
- 巻号頁・発行日
- vol.7, no.1, 2012-01-18
- 被引用文献数
- 33
Background: The transport of endoplasmic reticulum (ER)-derived COPII vesicles toward the ER-Golgi intermediatecompartment (ERGIC) requires cytoplasmic dynein and is dependent on microtubules. p150<sup>Glued</sup>, a subunit of dynactin, hasbeen implicated in the transport of COPII vesicles via its interaction with COPII coat components Sec23 and Sec24. However,whether and how COPII vesicle tether, TRAPP (Transport protein particle), plays a role in the interaction between COPIIvesicles and microtubules is currently unknown.Principle Findings: We address the functional relationship between COPII tether TRAPP and dynactin. Overexpressed TRAPPsubunits interfered with microtubule architecture by competing p150<sup>Glued</sup> away from the MTOC. TRAPP subunit TRAPPC9bound directly to p150<sup>Glued</sup> via the same carboxyl terminal domain of p150<sup>Glued</sup> that binds Sec23 and Sec24. TRAPPC9 alsoinhibited the interaction between p150<sup>Glued</sup> and Sec23/Sec24 both in vitro and in vivo, suggesting that TRAPPC9 serves touncouple p150<sup>Glued</sup> from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane.Conclusions: These findings provide a new perspective on the function of TRAPP as an adaptor between the ERGICmembrane and dynactin. By preserving the connection between dynactin and the tethered and/or fused vesicles, TRAPPallows nascent ERGIC to continue the movement along the microtubules as they mature into the cis-Golgi.
1 0 0 0 OA 細胞内輸送の高効率化とゴルジ体構造変化に関わるゴルジンタンパク質の機能解析
これまでに、ゴルジンタンパク質群のひとつであるgiantinをノックダウンすると分泌が上がること、ゴルジ体の構造に変化が起こることを示した。本研究課題では、この現象に関わる分子機構を明らかにすることを最終的な目的とし、電子線トモグラフィーなどを用いゴルジ体の構造を詳細に解析した。また、この間、分泌調節に関わる新規小分子を発見したため、新規分子の特徴付けを合わせて行なった。本研究課題の期間は終了したが、引き続き、これらの解析や新規分子のターゲットの探索を行う予定である。