著者
清水 茂雅 堀口 明日香 山崎 浩司 川合 祐史
出版者
北海道大学大学院水産科学研究院 = Research Faculty of Fisheries Sciences, Hokkaido University
雑誌
北海道大学水産科学研究彙報 (ISSN:13461842)
巻号頁・発行日
vol.59, no.2, pp.37-42, 2009-08-03

Recovery of heat-injured vegetative cells and spores of Clostridium perfringens was evaluated on selective media, CW Egg-yolk agar with kanamycin (ECW+A), Tryptose sulfite cycloserine agar(TSC+A)and modified Handford agar (mHFA). As a result of heat treatment at 54℃ for 15 min, viable counts of C. perfringens vegetative cells on a selective medium (ECW+A)was significantly less than those on non-selective media. This means C. perfringens vegetative cells should be in an injured state. Comparing of the three selective media, TSC+A was the best growth and recovery of heat-injured C. perfringens vegetative cells. Recovery on ECW+A was 1 log CFU/ml less than that on TSC+A. Supplementation of TSC+A with sodium pyruvate (0.1-0.5%) further enhanced recovery and detection of heat-injured C. perfringens vegetative cells, and its efficacy was the highest on TSC+A supplemented with 0.3% sodium pyruvate. Supplementation of TSC+A with sodium pyruvate did not affect recovery of heat-injured (95℃, 2 or 60 min) C. perfringens spores. These findings suggest that TSC+A is the most favorable medium for enumeration of C. perfringens and supplementation with sodium pyruvate improves recovery and detection of heat-injured C. perfringens.

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嫌気性菌の生菌数ですが、 クロストの生菌数ならば、--- 下記を参照にしてください。 嫌気性菌用培地はありますか?(クロストリジュウム用) 嫌気環境をつくる必要があります。嫌気性パック、発生袋、嫌気性用希釈培地 -- http://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/39007/1/59-2_001.pdf

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