著者

Shoma MIKAWA Yuichi MIYAGAWA Noriko TODA Yoshinori TOMINAGA Naoyuki TAKEMURA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0050, (Released:2014-10-16)

被引用文献数

1 5

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Pulmonary hypertension (PH) often occurs due to a left heart disease such as myxomatous mitral valve disease (MMVD) in dogs and is diagnosed using Doppler echocardiography and estimated pulmonary arterial pressure. Diagnosis of PH in dogs requires expertise in echocardiography: however, the examination for PH is difficult to perform in a clinical setting. Thus, simple and reliable methods are required for the diagnosis of PH in dogs. The purpose of this study was to develop models using multiple logistic regression analysis to detect PH due to left heart disease in dogs with MMVD without echocardiography. The medical records of dogs with MMVD were retrospectively reviewed, and 81 dogs were included in this study and classified into PH and non-PH groups. Bivariate analysis was performed to compare all parameters between the groups, and variables with P values of <0.25 in bivariate analysis were included in multiple logistic regression analysis to develop models for the detection of PH. In multiple logistic regression analysis, the model included a vertebral heart scale short axis of >5.2 v, and a length of sternal contact of >3.3 v was considered suitable for the detection of PH. The predictive accuracy of this model (85.9%) was judged statistically adequate, and therefore, this model may be useful to screen for PH due to left heart disease in dogs with MMVD without echocardiography.

著者

Reiko USUI Yuki OKADA Emiko FUKUI Atsuhiko HASEGAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0072, (Released:2014-10-16)

被引用文献数

1 5

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Otitis media of the left ear was diagnosed by video otoscopic examination in a 7-year-old, intact male Shih-tzu dog (weight, 5.1 kg), that also had three complex ceruminous adenomas and a Pseudomonas aeruginosa infection in the left ear canal. In such cases, total ear canal ablation is usually required. However, a complete cure was achieved in the present case without total ear canal ablation. The complex ceruminous adenomas were excised using a diode laser, and repeated cleansing of the tympanic cavity and ear canal was implemented using a video otoscope. As a result, the ear canal was closed in a U-form, and the otitis media was cured.

著者

Zengyang PEI Jinqiu WANG

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0212, (Released:2014-10-14)

被引用文献数

2 11

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Sepsis is a major cause of mortality in intensive care medicine. Propofol, an intravenous general anesthetic, has been suggested to have anti-inflammatory properties and able to prevent sepsis induced by Gram-positive and Gram-negative bacteria by down-regulating the gene expression of pro-inflammatory cytokines. However, propofol’s anti-inflammatory effects upon canine peripheral blood mononuclear cells (PBMCs) have not yet been clarified. Here, we isolate canine PBMCs and investigate the effects of propofol on the gene expressions of both lipopolysaccharide (LPS)-induced interleukin-6 (IL-6) and tumor necrosis factor (TNF)-α and upon the production of nitric oxide (NO). Through real-time quantitative PCR and the Griess reagent system, we found that non-cytotoxic levels of propofol significantly inhibited the release of NO and IL-6 and TNF-α gene expression in LPS-induced canine PBMCs. Western blotting revealed that LPS does significantly increase the expression of inducible NO synthase (iNOS) protein in canine PBMCs, while pretreatment with propofol significantly decreases the LPS-induced iNOS protein expression. Propofol, at concentration of 25 µM and 50 µM, also significantly inhibited the LPS-induced nuclear translocation of nuclear factor (NF)-κB p65 protein in canine PBMCs. This diminished TNF-α, IL-6 and iNOS expression and NO production was in parallel to the respective decreased NF-κB p65 protein nuclear translocation in the LPS-activated canine PBMCs pretreated with 25 µM and 50 µM propofol. This suggests that non-cytotoxic levels of propofol pretreatment can down-regulate LPS-induced inflammatory responses in canine PBMCs, possibly by inhibiting the nuclear translocation of the NF-κB p65 protein.

著者

Rei NAKANO Kazuya EDAMURA Tomohiro NAKAYAMA Kenji TESHIMA Kazushi ASANO Takanori NARITA Ken OKABAYASHI Hiroshi SUGIYA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0284, (Released:2014-10-06)

被引用文献数

1 21

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We investigated the in vitro differentiation of canine bone marrow stromal cells (BMSCs) into voltage- and glutamate-responsive neuron-like cells. BMSCs were obtained from the bone marrow of healthy beagle dogs. Canine BMSCs were incubated with the basal medium for neurons containing recombinant human basic fibroblast growth factor (bFGF; 100 ng/ml). The viability of the bFGF-treated cells was assessed by a trypan blue exclusion assay, and the morphology was monitored. Real-time RT-PCR was performed to evaluate mRNA expression of neuronal, neural stem cell and glial markers. Western blotting and immunocytochemical analysis for the neuronal markers were performed to evaluate the protein expression and localization. The Ca2+ mobilization of the cells was evaluated using the Ca2+ indicator Fluo3 to monitor Ca2+ influx. To investigate the mechanism of bFGF-induced neuronal differentiation, the fibroblast growth factor receptor inhibitor, the phosphoinositide 3-kinase inhibitor or the Akt inhibitor was tested. The bFGF treatment resulted in the maintenance of the viability of canine BMSCs for 10 days, in the expression of neuronal marker mRNAs and proteins and in the manifestation of neuron-like morphology. Furthermore, in the bFGF-treated BMSCs, a high concentration of KCl and L-glutamate induced an increase in intracellular Ca2+ levels. Each inhibitor significantly attenuated the bFGF-induced increase in neuronal marker mRNA expression. These results suggest that bFGF contributes to the differentiation of canine BMSCs into voltage- and glutamate-responsive neuron-like cells and may lead to the development of new cell-based treatments for neuronal diseases.

著者

Tae-Ho CHUNG Catherine LAMM Young-Chul CHOI Jung-Woo LEE Dohyeon YU Ul-Soo CHOI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0088, (Released:2014-10-03)

被引用文献数

1 4

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A 7-month-old castrated male French Bull dog was presented with vomiting, lethargy, anorexia and weight loss of 2 weeks duration. The patient’s history and clinical manifestations of suspected hepatopathy were subjected to ultrasonography, radiography, biochemical investigations and cytology of hepatic lesion. The cytologic impression was hepatic lymphoma, which was later confirmed by histopathology. The neoplastic cells were strongly diffusely immunoreactive for PAX5, but not immunoreactive for CD3, and B lymphocyte specific clonal proliferation was detected using by assay of antigen receptor rearrangement. Large numbers of immunoreactive mature non-neoplastic lymphocytes were admixed with the neoplastic cell population. Therefore, the immunohistochemical results were definitively consistent with a T-cell rich B-cell lymphoma (TCRBCL). This is the first description of a hepatic TCRBCL in a juvenile dog showing a poor response to aggressive chemotherapy.

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.5, pp.293-299, 1936-04-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.11, pp.690-692, 1936-09-20 (Released:2008-10-24)

著者

池田 錫

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.9, pp.529-533, 1936-07-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.11, no.1, pp.25-32, 1938-01-20 (Released:2008-10-24)

被引用文献数

1

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.10, no.13, pp.743-748, 1937-11-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.12, pp.759-768, 1936-10-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.10, no.14, pp.825-830, 1937-12-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.10, pp.614-617, 1936-08-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.9, pp.534-538, 1936-07-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.9, no.7, pp.446-450, 1936-06-20 (Released:2008-10-24)

著者

中澤 壽三郎

出版者

公益社団法人 日本獣医学会

雑誌

應用獸醫學雑誌 (ISSN:1883907X)

巻号頁・発行日

vol.10, no.12, pp.683-687, 1937-10-20 (Released:2008-10-24)

著者

Kikumi OGIHARA Ken ONDA Reiichiro SATO Yuko NAYA Hideharu OCHIAI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0014, (Released:2014-09-23)

被引用文献数

1 4

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L-type amino acid transporter 1 (LAT1), the first isotype of amino acid transport system L, transports aromatic and branched amino acids pivotal for fundamental cellular activities such cellular growth and proliferation. LAT1 expression was high only in the brain in contrast to its limited distribution and low level of expression in normal tissues. We found potent LAT1 expression in canine caput epididymis by quantitative RT-PCR and Western blotting analysis. Immnuno-histochemical examination revealed observable LAT1 in microvillous epithelial cells.

著者

Shun HIRASAWA Miki SHIMIZU Yuumi MARUI Miori KISHIMOTO Seiichi OKUNO

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0089, (Released:2014-09-05)

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We designed a new method of measuring the length of the ulnar nerve and determining standard values for F-wave parameters of the ulnar nerve in clinically normal beagles. Nerve length must be precisely measured to determine F-wave latency and conduction velocity. The length of the forelimb has served as the length of the ulnar nerve for F-wave assessments, but report indicates that F-wave latency is proportional to the length of the pathway traveled by nerve impulses. Therefore, we measured the surface distance from a stimulus point to the spinous process of the first thoracic vertebra (nerve length 1) and the anterior horn of the scapula (nerve length 2) as landmarks through the olecranon and the shoulder blade acromion. The correlation coefficients between the shortest F-wave latency and the length of nerves 1, 2 or the forelimb were 0.61, 0.7 and 0.58. Nerve length 2 generated the highest value. Furthermore, the anterior horn of the scapula was easily palpated in any dog regardless of well-fed body. We concluded that nerve length 2 was optimal for measuring the length of the ulnar nerve.

著者

Shintaro TOMURA Mona UCHIDA Tomohiro YONEZAWA Masato KOBAYASHI Makoto BONKOBARA Satoko ARAI Toru MIYAZAKI Satoshi TAMAHARA Naoaki MATSUKI

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.14-0166, (Released:2014-09-01)

被引用文献数

1 5

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Apoptosis inhibitor of macrophage (AIM) plays roles in survival of macrophages. In this study, we cloned canine AIM cDNA and observed its transcriptional expression levels in various tissues. The coding sequence of canine AIM was 1,023 bp encoding 340 amino acid residues, which had around 65% homology with those of the human, mouse and rat. Transcriptional expression of AIM was observed in the spleen, lung, liver and lymph node, which confirmed the expression of canine AIM in tissue macrophages. Moreover, AIM was highly expressed in one of the canine histiocytic sarcoma cell lines. CD36, the receptor of AIM, was also expressed in various tissues and these cell lines. These findings are useful to reveal the actual functions of canine AIM.

著者

Shunsuke SHIMAMURA Ken KIMURA Masaaki KATAYAMA Tadahisa MASHITA Kenichi MAEDA Saori KOBAYASHI Reeko SATO Jun YASUDA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.13-0541, (Released:2014-08-21)

被引用文献数

4

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This study evaluated the number and function of neutrophils during 3 hr of hemodialysis in healthy dogs under anesthesia. Isolated neutrophils were used to assess neutrophil adhesion, phagocytosis and the oxidative burst. At 0.5 and 3 hr after the start of hemodialysis treatment, there was a decrease in neutrophil number. The phagocytic ability of neutrophils was decreased 3 hr after the start of hemodialysis. In conclusion, this study demonstrated that hemodialysis reduces the number and phagocytic ability of neutrophils during treatment. However, these changes recover within 24 hr of hemodialysis.