1 0 0 0 OA 飼育用水の中圧紫外線処理によるヒラメスクーチカ症の防除
- 著者
- 笠井 久会 大沢 秀一 小林 正 吉水 守
- 出版者
- 日本魚病学会 = Japanese Society of Fish Pathology
- 雑誌
- 魚病研究 (ISSN:0388788X)
- 巻号頁・発行日
- vol.37, no.4, pp.199-200, 2002-12
Treatment of supplied water with a high quality ultraviolet (UV) lamp was examined for prevention of scuticociliatosis of farmed juvenile Japanese flounder (Paralichthys olivaceus). In an examination for the ciliate-cidal effect of UV irradiation, scuticociliates showed low susceptibility to UV compared with fish pathogenic viruses or bacteria, the minimal killing dosage being 2.0 × 10^5 μW・sec/cm^2. UV treatment of supplied water to the tank was performed in a flounder farm in southern Hokkaido, where scuticociliatosis frequently occurred. It was revealed that the UV treatment at 3.0 × 10^5 μW・sec/cm^2 was effective to prevent scuticociliatosis.
1 0 0 0 IR 口白症感染耐過トラフグ血清を用いた口白症関連タンパク質の検出
- 著者
- 高見 生雄 粉川 愉記 西澤 豊彦 吉水 守
- 出版者
- 日本魚病学会 = Japanese Society of Fish Pathology
- 雑誌
- 魚病研究 (ISSN:0388788X)
- 巻号頁・発行日
- vol.42, no.1, pp.29-34, 2007-03-15
- 被引用文献数
- 2 2
Kuchijirosho (snout ulcer disease) is an infectious disease of tiger puffer Takifugu rubripes in Japan and Korea. Little is known about detailed characteristics on the causative agent of kuchijirosho, except it is a filterable agent. In the present study, kuchijirosho associated proteins (KAPs) with molecular weight of 100-120 kDa were detected in brain tissues of tiger puffer by western blot analysis with sera of tiger puffer which survived kuchijirosho, but not in the kidney or spleen tissues. Interestingly, KAPs existed in brain tissues of tiger puffer with no history of kuchijirosho, however, the KAPs were not recognized by those healthy fish sera. KAPs were fractionated in supernatant of brain tissue homogenate containing the kuchijirosho agent by ultra-centrifugation, while pathogenicity of the kuchijirosho agent was observed in the precipitated fraction rather than the supernatant. These results suggest that KAPs are not structural proteins of the kuchijirosho agent, and could be brain tissue proteins antigenically altered by infection of the kuchijirosho agent. It is also considered that the causative agent could be low in antigenicity to host fish because structural proteins of the causative agent was never recognized by host fish sera.