著者

Fukuda Yuuki Ando Yasuhiro

出版者

Japanese Society of Fisheries Science

雑誌

Fisheries Science (ISSN:09199268)

巻号頁・発行日

vol.77, no.5, pp.875-882, 2011-09

被引用文献数

4

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Fatty acid analysis of roughscale sole Clidoderma asperrimum flesh lipids was carried out by gas chromatography. An unidentified peak appeared in the chromatogram in the elution region of ≥C24 fatty acids. After enrichment by solvent partitioning, reversed-phase thin-layer chromatography (TLC), and argentation TLC, the peak component was subjected to structural analyses. The partially hydrogenated products after reaction with hydrazine hydrate gave seven isomers of cis-hexacosenoic acid (26:1). Gas chromatography-mass spectrometry (GC–MS) analyses of their dimethyl disulfide adducts identified the monounsaturates as 5-, 8-, 11-, 14-, 17-, 20-, and 23-26:1. The peak component was assigned to all-cis-5,8,11,14,17,20,23-hexacosaheptaenoic acid (26:7n-3). GC–MS analyses of the 4,4-dimethyloxazoline derivative and methyl ester confirmed this structure. This fatty acid is a rare, very long-chain polyunsaturated fatty acid (VLCPUFA). The concentrations of the acid found in roughscale sole were 0.69 ± 0.34% (N = 5) of total fatty acids in flesh lipids. Roughscale sole appears to be characterized by the occurrence of 26:7n-3, which is lacking in popular sources of methylene-interrupted VLCPUFA, such as vertebrate retina, spermatozoa, and herring.

著者

Okumura Sei-ichi Arai Katsutoshi Harigaya Yoshitaka Eguchi Hirotaka Sakai Mizuho Senbokuya Hiroaki Furukawa Suehiro Yamamori Kunio

出版者

Japanese Society of Fisheries Science

雑誌

Fisheries Science (ISSN:09199268)

巻号頁・発行日

vol.73, no.2, pp.237-243, 2007

被引用文献数

11

著者

Awano Shinichi Miyamoto Koji Hosokawa Masashi Mankura Mitsumasa Takahashi Koretaro

出版者

Japanese Society of Fisheries Science

雑誌

Fisheries Science (ISSN:09199268)

巻号頁・発行日

vol.72, pp.909-912, 2006

被引用文献数

11