著者

松原 茂規 三鴨 廣繁 山岸 由佳

出版者

日本耳鼻咽喉科感染症・エアロゾル学会

雑誌

日本耳鼻咽喉科感染症・エアロゾル学会会誌 (ISSN:21880077)

巻号頁・発行日

vol.4, no.2, pp.107-115, 2016-05-20 (Released:2020-09-03)

参考文献数

10

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We report here that we could simultaneously detect many species of viruses (adenovirus, influenza virus, parainfluenza virus, rhinovirus, respiratory syncytial (RS) virus, bocavirus, metapneumovirus, coronavirus and enterovirus) among a total of 242 patients (121 males and 121 females; 4 of these patients were tested twice to make samples) with acute upper respiratory tract inflammation who visited our hospital between January 2015 and March 2015, by using RV 16 detection kit (AnyplexTM II RV16 detection kit, Seegene, South Korea) based on real-time PCR system. The mean age for these patients was 16.5 years, with a range of 4 months to 90 years. Nasopharyngeal samples from all of the patients were used for virus tests, and nasopharyngeal samples from patients suspected to have bacterial infection were used for bacteria tests. Virus speciemens were refrigerated and sent to Microbiological Laboratory, Department of Infection Control and Prevention, Aichi Medical University for analysis.Only viruses were detected in 153 patients (63%), viruses and bacteria were detected in 63 patients (26%), only bacteria were detected in 14 patients (6%), and neither viruses nor bacteria was detected in 12 patients (5%). The number of virus species detected per patient ranged from zero to six, 1.9 species on average. Adenovirus was the most frequently detected virus, followed by coronavirus, rhinovirus, metapneumovirus, and bocavirus. Adnovirus, coronavirus and influenza virus were commonly detected in January, metapneumovirus was frequently detected in February and March, and rhinovirus and bocavirus were common in March. Among the 242 patients, there were 18 patients with acute otitis media, 15 with exudative otitis media, 42 with acute rhinosinusitis and 14 with acute nasopharyngitis. Adnovirus, coronavirus, rhinovirus and metapneumovirus were often detected in patients with acute otitis media and those with exudative otitis media. In addition to these viruses, bocavirus was also frequently detected in patients with acute rhinosinusitis. Haemophilus influenzae or Moraxella catarrhalis and Adenovirus, rhinovirus, bocavirus, metapneumovirus, or coronavirus has been concurrently detected at a rate of 50% or more in patients with acute otitis media, exudative otitis media, acute rhinosisusitis and acute nasopharyngitis. Streptococcus pneumoniae and metapneumovirus or coronavirus has been concurrently detected in many cases.We consider that testing for detecting viruses causing upper respiratory tract infection will be useful for understanding the pathological condition of each patient, infection control in the community and detected causal microorganisms in patients with increasing severity of illness, if such testing is routinely performed in the future.