著者
Ken-ichiro Taoka Zenpei Shimatani Koji Yamaguchi Mana Ogawa Hiromi Saitoh Yoichi Ikeda Hiroko Akashi Rie Terada Tsutomu Kawasaki Hiroyuki Tsuji
出版者
Japanese Society for Plant Biotechnology
雑誌
Plant Biotechnology (ISSN:13424580)
巻号頁・発行日
pp.20.1209a, (Released:2021-03-11)
参考文献数
44

Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (Oryza sativa). In both plant-based two-hybrid and split luc complementation (SLC) assays, Nluc activity was detected with higher sensitivity and specificity than that with Fluc. To apply Nluc to research involving the photoperiodic regulation of flowering, we made a knock-in rice plant in which the Nluc coding region was inserted in-frame with the OsMADS15 gene, a target of the rice florigen Hd3a. Strong Nluc activity in response to Hd3a, and in response to change in day length, was detected in rice protoplasts and in a single shoot apical meristem, respectively. Our results indicate that Nluc assay systems will be powerful tools to monitor gene expression and protein-protein interaction in plant research.

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外部データベース (DOI)

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凄い明るさのNanoLucによる新しい実験系の論文を発表しました!従来の実験系で見えないものが見えます。ハイスループットなスクリーニング系への展開も期待できる。特任助教の田岡さんの力作。精密単離したメリステム一個でLuc活性測定するなど、特別な技術を開発しています https://t.co/Kj29loi9LH

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