- 著者
- Ken-ichiro Taoka Zenpei Shimatani Koji Yamaguchi Mana Ogawa Hiromi Saitoh Yoichi Ikeda Hiroko Akashi Rie Terada Tsutomu Kawasaki Hiroyuki Tsuji
- 出版者
- Japanese Society for Plant Biotechnology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- pp.20.1209a, (Released:2021-03-11)
- 参考文献数
- 44
- 被引用文献数
- 7
Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (Oryza sativa). In both plant-based two-hybrid and split luc complementation (SLC) assays, Nluc activity was detected with higher sensitivity and specificity than that with Fluc. To apply Nluc to research involving the photoperiodic regulation of flowering, we made a knock-in rice plant in which the Nluc coding region was inserted in-frame with the OsMADS15 gene, a target of the rice florigen Hd3a. Strong Nluc activity in response to Hd3a, and in response to change in day length, was detected in rice protoplasts and in a single shoot apical meristem, respectively. Our results indicate that Nluc assay systems will be powerful tools to monitor gene expression and protein-protein interaction in plant research.
- 著者
- Ken-ichiro Taoka Zenpei Shimatani Koji Yamaguchi Mana Ogawa Hiromi Saitoh Yoichi Ikeda Hiroko Akashi Rie Terada Tsutomu Kawasaki Hiroyuki Tsuji
- 出版者
- Japanese Society for Plant Biotechnology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.38, no.1, pp.89-99, 2021-03-25 (Released:2021-03-25)
- 参考文献数
- 44
- 被引用文献数
- 7
Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (Oryza sativa). In both plant-based two-hybrid and split luc complementation (SLC) assays, Nluc activity was detected with higher sensitivity and specificity than that with Fluc. To apply Nluc to research involving the photoperiodic regulation of flowering, we made a knock-in rice plant in which the Nluc coding region was inserted in-frame with the OsMADS15 gene, a target of the rice florigen Hd3a. Strong Nluc activity in response to Hd3a, and in response to change in day length, was detected in rice protoplasts and in a single shoot apical meristem, respectively. Our results indicate that Nluc assay systems will be powerful tools to monitor gene expression and protein-protein interaction in plant research.
- 著者
- Mana Ogawa Satoko Hoshino Motoko Fujiwara Hiroki Nakata
- 出版者
- The Japanese Society of Physical Fitness and Sports Medicine
- 雑誌
- The Journal of Physical Fitness and Sports Medicine (ISSN:21868131)
- 巻号頁・発行日
- vol.8, no.3, pp.127-136, 2019-05-25 (Released:2019-05-18)
- 参考文献数
- 23
- 被引用文献数
- 1 4
We investigated the relationship between basketball free-throw accuracy and anthropometry, physical fitness tests, and performance variables among 16 collegiate female basketball players. Each participant performed 20 basketball free throws. Anthropometric measures were height and weight; physical fitness tests were sit-and-reach, back strength, and grip strengths; other basketball performance variables were the phases of the pre-shoot routine: (a) time taken, (b) minimum angle when taking the ball back, (c) angle at ball release, (d) angular displacement during the forward arm swing, and (e) angular velocity at ball release on the elbow, shoulder, hip, knee, and ankle. We analyzed the correlation between free-throw accuracy and data on anthropometry and physical fitness, the time period and variability of the pre-shot routine, and kinematic data. There were negative correlations between free-throw accuracy and mean pre-shot time, and variability of the pre-shot time, indicating that participants with a shorter and less variable pre-shot time showed a higher free-throw accuracy. Angular displacement of the shoulder during the forward swing and angular velocity of the knee at ball release showed positive correlations with free-throw accuracy. There was also a negative correlation between free-throw accuracy and variability of angular displacement during the forward swing at the elbow, indicating that participants with smaller variability of angular displacement of the elbow showed higher free-throw accuracy. Some performance variables, including routine duration, angular displacement of the shoulder and elbow, and angular velocity of the knee, were related to free-throw accuracy.