著者

Ken-ichiro Taoka Zenpei Shimatani Koji Yamaguchi Mana Ogawa Hiromi Saitoh Yoichi Ikeda Hiroko Akashi Rie Terada Tsutomu Kawasaki Hiroyuki Tsuji

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.20.1209a, (Released:2021-03-11)

参考文献数

44

被引用文献数

7

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Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (Oryza sativa). In both plant-based two-hybrid and split luc complementation (SLC) assays, Nluc activity was detected with higher sensitivity and specificity than that with Fluc. To apply Nluc to research involving the photoperiodic regulation of flowering, we made a knock-in rice plant in which the Nluc coding region was inserted in-frame with the OsMADS15 gene, a target of the rice florigen Hd3a. Strong Nluc activity in response to Hd3a, and in response to change in day length, was detected in rice protoplasts and in a single shoot apical meristem, respectively. Our results indicate that Nluc assay systems will be powerful tools to monitor gene expression and protein-protein interaction in plant research.

著者

Ken-ichiro Taoka Zenpei Shimatani Koji Yamaguchi Mana Ogawa Hiromi Saitoh Yoichi Ikeda Hiroko Akashi Rie Terada Tsutomu Kawasaki Hiroyuki Tsuji

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.1, pp.89-99, 2021-03-25 (Released:2021-03-25)

参考文献数

44

被引用文献数

7

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Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (Oryza sativa). In both plant-based two-hybrid and split luc complementation (SLC) assays, Nluc activity was detected with higher sensitivity and specificity than that with Fluc. To apply Nluc to research involving the photoperiodic regulation of flowering, we made a knock-in rice plant in which the Nluc coding region was inserted in-frame with the OsMADS15 gene, a target of the rice florigen Hd3a. Strong Nluc activity in response to Hd3a, and in response to change in day length, was detected in rice protoplasts and in a single shoot apical meristem, respectively. Our results indicate that Nluc assay systems will be powerful tools to monitor gene expression and protein-protein interaction in plant research.

著者

Yuma ABE Masaki OGURA Hiroyuki TSUJI Amane MIURA Shuichi ADACHI

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE TRANSACTIONS on Fundamentals of Electronics, Communications and Computer Sciences (ISSN:09168508)

巻号頁・発行日

vol.E103-A, no.2, pp.492-501, 2020-02-01

被引用文献数

5

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Satellite communications (SATCOM) systems play important roles in wireless communication systems. In the future, they will be required to accommodate rapidly increasing communication requests from various types of users. Therefore, we propose a framework for efficient resource management in large-scale SATCOM systems that integrate multiple satellites. Such systems contain hundreds of thousands of communication satellites, user terminals, and gateway stations; thus, our proposed framework enables simpler and more reliable communication between users and satellites. To manage and control this system efficiently, we formulate an optimization problem that designs the network structure and allocates communication resources for a large-scale SATCOM system. In this mixed integer programming problem, we allow the cost function to be a combination of various factors so that SATCOM operators can design the network according to their individual management strategies. These factors include the total allocated bandwidth to users, the number of satellites and gateway stations to be used, and the number of total satellite handovers. Our numerical simulations show that the proposed management strategy outperforms a conventional strategy in which a user can connect to only one specific satellite determined in advance. Furthermore, we determine the effect of the number of satellites in the system on overall system performance.