著者

NISHIOKA Midori SUMIDA Masayuki OHTA Shigeru SUZUKI Hiroshi

出版者

広島大学

雑誌

Scientific report of the Laboratory for Amphibian Biology (ISSN:03863166)

巻号頁・発行日

vol.9, pp.53-96, 1987-12

被引用文献数

3

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Speciation of three allied genera, Buergeria, Rhacophorus and Polypedates, distributed in the Far East, was biochemically examined by the electrophoretic method. Electrophoretic patterns of 16 enzymes extracted from the skeletal muscles and livers and three blood proteins were analyzed in 257 frogs belonging to 14 populations, including one of Buergeria buergeri, four of B. japonica, two of Rhacophorus arboreus, two of Rh. schlegelii, three of Rh. viridis, one of Rh. taipeianus and one of Polypedates leucomystax. These frogs had 25 loci in total. While 10 enzymes, ADA, ADH, AK, CK, Fum, α-GDH, GPI, ME, MPI and PGM, and three blood proteins, Ab, Prot-C and Hb, had a single locus, the other six enzymes, AAT, IDH, LDH, MDH, Pep and SOD, had two loci. At each of the 25 loci, there were 3∿33 phenotypes, 10.6 on the average, produced by 3∿17 alleles, 7.5 on the average. The mean proportions of heterozygous loci per individual in each of the 10 populations of Buergeria and Rhacophorus which were 5∿58 in sample size were 4.0∿23.0%, 12.0% on the average, when examined at the 25 loci controlling 16 enzymes and three blood proteins. These 10 populations were 24∿68%, 44.8% on the average, in mean proportion of polymorphic loci per population and 1.28∿2.12,1.64 on the average, in mean number of alleles per locus. Genetic distances were estimated from gene frequencies by the method of NEI (1972,1975). Those among different populations of B. japonica, Rh. arboreus and Rh. schlegelii were 0.003∿0.270,and those among different subspecies of Rh. viridis were 0.277∿0.865. The genetic distances among four species, Rh. arboreus, Rh. schlegelii, Rh. viridis and Rh. taipeianus, were 0.301∿0.854,while those between Buergeria buergeri and four populations of B. japonica were 2.045∿2.243. The genetic distances between Polypedates and Buergeria and between Polypedates and Rhacophorus were 3.073∿4.572 and 1.183∿1.445,respectively. The genetic distances between Buergeria and Rhacophorus were very large, being from 2.782 to unlimited number owing to nonexistence of common alleles between them. A dendrogram was drawn for the species and populations of Buergeria, Rhacophorus and Polypedates on the basis of genetic distances by the UPGMA clustering method (SNEATH and SOKAL, 1973; NEI, 1975).