The purposes of this study are to construct the College Life Anxiety Scale for measuring the level of college students' anxiety and to examine its reliability and validity. After collecting items about the anxiety in college life, factor analysis was performed on the data obtained from 2 782 college students. The result, firstly, showed that the College Life Anxiety Scale consisting of 30 items had three factors, which were daily life anxiety, test anxiety and college maladjustment. Secondly, it was indicated that the Scale had both high test-retest reliability and internal consistency (.82 and .84, respectively). It was also suggested that the Scale had high content, clinical, and criterion-related validities. In conclusion, the College Life Anxiety Scale is well able to measure the level of college students' anxiety in their usual life.