著者
山口 昌樹 花輪 尚子 吉田 博
出版者
社団法人日本生体医工学会
雑誌
生体医工学 : 日本エム・イー学会誌 (ISSN:1347443X)
巻号頁・発行日
vol.45, no.2, pp.161-168, 2007-06-10
参考文献数
26
被引用文献数
28

In order to realize a novel handheld monitor for the sympathetic nervous system, we fabricated an analytical system for salivary amylase activity (sAMY) using a dry-chemistry system called a"Cocoro Meter." The device to quantify sAMY using an activity rate method was completely automated. This method was made possible by the fabrication of a disposable test-strip equipped with built-in salivacollecting and reagent papers, and an automatic saliva transfer mechanism. Within a range of sAMY between 0-200 kU/l, the calibration curve for the monitor showed a coefficient of R^2=0.988 and CV (coefficient of variation) of 10.2%. The reproducibility between devices was within 10%. Moreover, it was demonstrated that : (i) the quantitativity of the sample collected by the test-strip was sufficient, (ii) the sublingual area was suitable as the sampling site of the saliva, and (iii) 30 s was sufficient for saliva sampling. Considering all of these effects including the saliva sampling site, a 12.1% CV was obtained for this monitor. A total of 1 min was sufficient to analyze the sAMY. Thus, this study demonstrated that the monitor might be used as a good index for psychological research.

言及状況

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@drcn79 アドレナリンβ受容体の刺激でアミラーゼの分泌が増えるらしい。消化管運動は副交感神経と習っているので意外な気はした。 参考:https://t.co/4mOlCSAl7q

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