- 著者
-
山口 昌樹
花輪 尚子
吉田 博
- 出版者
- 社団法人日本生体医工学会
- 雑誌
- 生体医工学 : 日本エム・イー学会誌 (ISSN:1347443X)
- 巻号頁・発行日
- vol.45, no.2, pp.161-168, 2007-06-10
- 参考文献数
- 26
- 被引用文献数
-
28
In order to realize a novel handheld monitor for the sympathetic nervous system, we fabricated an analytical system for salivary amylase activity (sAMY) using a dry-chemistry system called a"Cocoro Meter." The device to quantify sAMY using an activity rate method was completely automated. This method was made possible by the fabrication of a disposable test-strip equipped with built-in salivacollecting and reagent papers, and an automatic saliva transfer mechanism. Within a range of sAMY between 0-200 kU/l, the calibration curve for the monitor showed a coefficient of R^2=0.988 and CV (coefficient of variation) of 10.2%. The reproducibility between devices was within 10%. Moreover, it was demonstrated that : (i) the quantitativity of the sample collected by the test-strip was sufficient, (ii) the sublingual area was suitable as the sampling site of the saliva, and (iii) 30 s was sufficient for saliva sampling. Considering all of these effects including the saliva sampling site, a 12.1% CV was obtained for this monitor. A total of 1 min was sufficient to analyze the sAMY. Thus, this study demonstrated that the monitor might be used as a good index for psychological research.