著者

花輪 尚子 才木 祐司 山口 昌樹

出版者

日本生理人類学会

雑誌

日本生理人類学会誌 (ISSN:13423215)

巻号頁・発行日

vol.13, no.1, pp.49-56, 2008-02-25 (Released:2017-07-28)

参考文献数

24

被引用文献数

2

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The purpose of this study is to evaluate the sedative effect of fragrances which have existed in Japan since long time ago such as sakura, ume and fuji. 17 healthy female Japanese subjects were enrolled (21.5±1.3yr). The salivary amylase activity (sAMY) was analyzed during the inhalation of fragrance. Using questionnaire, subjective evaluation was conducted. aAMY was significantly lower during inhalation of fragrance than control. Inhalation of fragrance affected subjects' work performance. Our results indicated that the Japanese fragrances induced the effect in Japanese females, as assessed by both analysis of sAMY and work performance.

著者

山口 昌樹 花輪 尚子 吉田 博

出版者

社団法人日本生体医工学会

雑誌

生体医工学 : 日本エム・イー学会誌 (ISSN:1347443X)

巻号頁・発行日

vol.45, no.2, pp.161-168, 2007-06-10

参考文献数

26

被引用文献数

28

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In order to realize a novel handheld monitor for the sympathetic nervous system, we fabricated an analytical system for salivary amylase activity (sAMY) using a dry-chemistry system called a"Cocoro Meter." The device to quantify sAMY using an activity rate method was completely automated. This method was made possible by the fabrication of a disposable test-strip equipped with built-in salivacollecting and reagent papers, and an automatic saliva transfer mechanism. Within a range of sAMY between 0-200 kU/l, the calibration curve for the monitor showed a coefficient of R^2=0.988 and CV (coefficient of variation) of 10.2%. The reproducibility between devices was within 10%. Moreover, it was demonstrated that : (i) the quantitativity of the sample collected by the test-strip was sufficient, (ii) the sublingual area was suitable as the sampling site of the saliva, and (iii) 30 s was sufficient for saliva sampling. Considering all of these effects including the saliva sampling site, a 12.1% CV was obtained for this monitor. A total of 1 min was sufficient to analyze the sAMY. Thus, this study demonstrated that the monitor might be used as a good index for psychological research.

著者

山口 昌樹 花輪 尚子 吉田 博

出版者

公益社団法人 日本生体医工学会

雑誌

生体医工学 (ISSN:1347443X)

巻号頁・発行日

vol.45, no.2, pp.161-168, 2007 (Released:2008-08-08)

参考文献数

26

被引用文献数

10

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In order to realize a novel handheld monitor for the sympathetic nervous system, we fabricated an analytical system for salivary amylase activity (sAMY) using a dry-chemistry system called a “Cocoro Meter.” The device to quantify sAMY using an activity rate method was completely automated. This method was made possible by the fabrication of a disposable test-strip equipped with built-in salivacollecting and reagent papers, and an automatic saliva transfer mechanism. Within a range of sAMY between 0-200 kU/l, the calibration curve for the monitor showed a coefficient of R2=0.988 and CV (coefficient of variation) of 10.2%. The reproducibility between devices was within 10%. Moreover, it was demonstrated that: (i) the quantitativity of the sample collected by the test-strip was sufficient, (ii) the sublingual area was suitable as the sampling site of the saliva, and (iii) 30 s was sufficient for saliva sampling. Considering all of these effects including the saliva sampling site, a 12.1% CV was obtained for this monitor. A total of 1 min was sufficient to analyze the sAMY. Thus, this study demonstrated that the monitor might be used as a good index for psychological research.