著者

林 正和 網野 比佐子 北 潔 村勢 則郎

出版者

低温生物工学会

雑誌

低温生物工学会誌 (ISSN:13407902)

巻号頁・発行日

vol.57, no.1, pp.87-90, 2011-04-15

参考文献数

8

被引用文献数

1

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Cryopreservation of the nematode Caenorhabditis elegans in the adult stage is of importance as the nematode is a powerful model organism. In this study, we applied the protocol previously established for cryopreservation of the L4 nematode to the adult one. Survival rate of 84% was obtained as a result. When ice nucleation active bacteria, Pseudomonas syringae, was used for the simplification of ice seeding procedure instead of the use of a pre-cooled needle, survival rate of 82% was obtained after thawing. Moreover, a simple method of cryopreservation using a deep freezer and polystyrene foam was developed for a practical purpose. According to the observation under a cryomicroscope, eggs in the nematode body were found to freeze at ca. -20℃ when cooled at the rate of 0.2℃/min after ice seeding at -3℃ although the nematode body remained unfrozen down to -100℃.