- 著者
-
長島 裕二
丸山 純一
野口 玉雄
橋本 周久
- 出版者
- 日本水産學會
- 雑誌
- 日本水産学会誌 (ISSN:00215392)
- 巻号頁・発行日
- vol.53, no.5, pp.819-823, 1987
- 被引用文献数
-
28
98
An ion-pairing high performance liquid chromatographic method using a silica ODS columnwas developed for analysis of paralytic shellfish poison (PSP) and tetrodotoxi (TTX). As mobile phase is used a mixture of 0.05<sub>M</sub> phosphate buffer (pH 7.0) containing 2 m<sub>M</sub> heptanesulfonicacid/methanol (99:1) for separation of gonyautoxins and TTXs, and the one (75:25) for separa-tion of saxitoxins. PSP and TTX members thus separated were detected as fluorogenic sub-stances, by heating with a periodate reagent (for PSP), or with 3<sub>N</sub> NaOH (for TTX).