著者
志村 悦子 沼知 健一 瀬崎 啓次郎 廣崎 芳次 渡部 終五 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.52, no.4, pp.725-730, 1986-04-25 (Released:2008-02-29)
参考文献数
11
被引用文献数
4 10

Four hybrids produced in an aquarium between the bottlenose dolphin Tursiops truncatus and the whitehead grampus Grampus griseus were analyzed electrophoretically to determine the genotypes coding to some enzymes and to ascertain the intergeneric hybridization in an artificial condition. The results obtained showed that each parental dolphin possessed its own gene (s) coding to each of the 3 enzymes (ICDH, GPI, MPI), and the hybrids had all the genes of the parental species. So was the case with an unidentified band detected on the sarcoplasmic protein pattern. The hybridization thus demonstrated was discussed in relation to the amount of differentiation and maintenance of species in the cetacean.
著者
カングスワン アタヤ 野口 玉雄 荒川 修 清水 潮 塚本 久美子 志田 保夫 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.54, no.10, pp.1799-1802, 1988-10-25 (Released:2008-02-29)
参考文献数
11
被引用文献数
6 11

Attempts were made to detect tetrodotoxin (TTX)-producing bacteria in the Thai horseshoe crab Carcinoscorpius rotundicauda, one of TTX-containing marine invertebrates. Thirteen dom-inant bacteria were isolated from the intestinal contents of live horseshoe crab specimens, and were cultured in a 2 × ORI medium at 20°C for 24h. Bacterial cells of each strain were harvested, ultrasonicated and ultrafiltered, and TTX and anh-TTX fractions were separated from the filtrate by Bio-Gel P-2 column chromatography. HPLC, UV and GC-MS analyses showed that all out of the 13 strains isolated produced anh-TTX, and one strain produced TTX and anh-TTX. The strain which produced both toxins was identified as Vibrio alginolyticus on the basis of biochemical and other characters.
著者
Attaya Kungsuwan 長島 裕二 野口 玉雄 志田 保夫 Sunee Suvapeepan Panthip Suwansakornkul 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.53, no.2, pp.261-266, 1987 (Released:2008-02-29)
参考文献数
14
被引用文献数
30 45

Further attempts were made to identify the toxins of the horseshoe crab Carcinoscorpius rotundicauda which causes sporadically food poisonings in Thailand. Toxic C. rotundicauda eggs were crushed and extracted with water repeatedly. After being defatted with dichloromethane, the combined extracts were ultrafiltered to cut off more than 1, 000 dalton-substances. Toxic fractions were obtained from the filtrate by column chromatography on Bio-Gel P-2 and Bio-Rex 70. Cellulose acetate strip electrophoresis, TLC, HPLC, and other instrumental analyses demon-strated that the horseshoe crab toxin was essentially composed of tetrodotoxin and related sub-stances, along with paralytic shellfish poisons as minor components.
著者
落合 芳博 渡部 終五 橋本 周久 成田 弘子 浮島 美之 奈良 正人
出版者
日本水産學會
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.50, no.4, pp.721-725, 1984
被引用文献数
5

Attempts were made to identify biochemically two gampylid fishes, escolar ("aburasokomutsu") <i>Lepidocybuum Flavobrunneum</i> and castor oil fish ("baramutsu") Ruvettus pretiosus, whose muscles contain a large amount of wax esters and hence may casuse a food poisoning when ingested. Myogen fractions were prepared from the ordinary muscles of both fishes as well as various fishes, and subjected to polyacrylamide gel electrophoresis, followed by staining with Coomassie brilliant bule for protein and with reagents for lactate and malate dehydrogenase activities. Results showed that the protein pattern is species-specific enougy to distinguish the two gempylid fishes from any of the other fishes tested. The pattern of either dehydrogenase was found to be less species-specific. However, simultaneous analyses of MDH and protein patterns faciliated the identification of buth gempylid fishes. Cellulose acetate membrane electrophophoresis was also found to be applicable for this purpose, though less satisfactorily.
著者
渕 祐一 野口 玉雄 斉藤 俊郎 森崎 澄江 仲摩 聡 嶋崎 晃次 林 薫 大友 信也 橋本 周久
出版者
公益社団法人 日本食品衛生学会
雑誌
食品衛生学雑誌 (ISSN:00156426)
巻号頁・発行日
vol.29, no.5, pp.320-324_1, 1988-10-05 (Released:2009-12-11)
参考文献数
9
被引用文献数
2 1

大分県地方のフグ料理専門店に伝えられていた, フグ肝臓の独特な調理方法による減毒機構について検討した. マウス試験及びHPLC, TLC, 電気泳動の結果から, 調理方法による減毒は, 手揉み工程ではフグ毒の溶出除去によること, また煮沸工程では加熱によって有毒肝臓中のテトロドトキシンがアンヒドロテトロドトキシン, さらにテトロドン酸へと順次構造変化を起こし, これに伴い毒性が弱毒性, 無毒性へと転換変化する現象に基づくことが明らかにされた. さらに, この煮沸工程での減毒には溶出による除去機構も関与していることが示唆された.
著者
岩本 宗昭 山中 英明 阿部 宏喜 渡部 終五 橋本 周久
出版者
公益社団法人 日本水産学会
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.56, no.1, pp.93-99, 1990-01-25 (Released:2008-02-29)
参考文献数
37
被引用文献数
20 29

Yellowtail, bartailed flathead and Japanese striped knifejaw were spiked at the brain, and stored at O°and 10°C. Yellowtail set in rigor-mortis after 2h, irrespective of storage temperature, attaining the full-rigor state after 7h at O°C and 15h at 10°C. Rigor-mortis of bartailed flathead started after 10h at O°C and 13h at 10°C. This species reached the full-rigor state after 20h at O°C and 40h at 10°C. In the case of Japanese striped knifejaw, rigor-mortis began to appear immediately after killing at O°C whereas after 4h at 10°C. The full-rigor state was attained with this fish after 13 and 20h, respectively, in the above order of storage temperature. Therefore, rigor-mortis proceeded roughly two times slower at 10°C than at O°C, regardless of fish species. In accordance with rigor-mortis progress, the muscle of each species showed a 2-3 times slower decrease of ATP concentration when stored at 10°C than when stored at O°C. Disappearance of creatine phosphate preceded that of ATP while lactate accumulation was nearly maximal when ATP disappeared, irrespective of fish species and storage temperature. No marked differences in initial ATP concentration were observed among the fish species examined. The highest lactate level was encountered in yellowtail which exhibited the fastest progress of rigor-mortis at either storage temperature. Japanese striped knifejaw showed a much lower accumulation of lactate than yellowtail, the accumulation of which was even lower than that in bartailed flathead. On the other hand, rigormortis progress of Japanese striped knifejaw was comparable to that of yellowtail and somewhat faster than that of bartailed flathead.
著者
畑江 敬子 松本 美鈴 島田 淳子 山中 英明 渡部 終五 橋本 周久
出版者
日本水産學會
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.56, no.9, pp.1521-1525, 1990
被引用文献数
5 2

The dorsal muscle of a carp was sliced into pieces 3mm thick, and six kinds of "arai" were prepared by washing the slices in water of regulated temperature for a specific time: 3 or 5 min in 0°C water, 70 s or 3 min in 18°C water, and 20 s or 70 s in 49°C water.<br> The physical property of "arai" was determined by 7 types of measurement. Factor analysis showed that physical properties, of all kinds of "arai" differed significantly from those of untreated fish slices and that "arai" at 0°C and 18°C treatments resembled each other. Treatment at 49°C yielded different results from the others. Sensory panel members could not discriminate the texture of 0°C and 18°C treatment, though they could discriminate 49°C treatment from the others. All of these samples at 0, 18, and 49°C treatment, were judged to be satisfactory.<br> After "arai" treatment, ATP content in the fish slices decreased; the higher the tem-perature of treatment, the more the ATP content decreased.<br> Scanning electron micrography, showed fewer lipid droplets on the surface of the "arai" slices than on that of untreated fish slices. "Arai" at 49°C treatment was smooth, so we presumed that there was thermal denaturation on the surface. Slight gaps between muscle fibers were found, which were not found in the case of untreated fish slices.
著者
野口 玉雄 橋本 周久
出版者
公益社団法人 日本農芸化学会
雑誌
化学と生物 (ISSN:0453073X)
巻号頁・発行日
vol.25, no.1, pp.44-52, 1987-01-25 (Released:2009-05-25)
参考文献数
23
被引用文献数
1 1 2
著者
長島 裕二 丸山 純一 野口 玉雄 橋本 周久
出版者
日本水産學會
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.53, no.5, pp.819-823, 1987
被引用文献数
28 94

An ion-pairing high performance liquid chromatographic method using a silica ODS columnwas developed for analysis of paralytic shellfish poison (PSP) and tetrodotoxi (TTX). As mobile phase is used a mixture of 0.05<sub>M</sub> phosphate buffer (pH 7.0) containing 2 m<sub>M</sub> heptanesulfonicacid/methanol (99:1) for separation of gonyautoxins and TTXs, and the one (75:25) for separa-tion of saxitoxins. PSP and TTX members thus separated were detected as fluorogenic sub-stances, by heating with a periodate reagent (for PSP), or with 3<sub>N</sub> NaOH (for TTX).