- 著者
- 上杉 薫 秋山 佳丈 浅野 豪文 秋山 義勝 大和 雅之 岡野 光夫 森島 圭祐
- 出版者
- 一般社団法人 日本機械学会
- 雑誌
- ロボティクス・メカトロニクス講演会講演概要集 2014 (ISSN:24243124)
- 巻号頁・発行日
- pp._3A1-I02_1-_3A1-I02_3, 2014-05-24 (Released:2017-06-19)
In this study, we measured mechanical properties of cell sheet by using uniaxial tensile test system which we developed. We applied kelvin model (viscoelastic model) which applied single cells for deriving mechanical properties such as elastic modulus and viscous modulus. Mechanical properties of Fibroblast (NIH-3T3) cell sheet and myoblast (C2C12) cell sheet were derived by fitting equation which related kelvin model to measured stress-strain curves of cell sheet. Furthermore, for confirming drag response on cell sheet mechanical properties. By deriving mechanical properties of cell sheet, we confirmed mechanical properties of cell sheet which are different from that of single cells.
- 著者
- 石橋 雄弥 浅野 豪文 上杉 薫 森島 圭祐
- 出版者
- 一般社団法人 日本機械学会
- 雑誌
- ロボティクス・メカトロニクス講演会講演概要集
- 巻号頁・発行日
- vol.2015, pp._1P1-O02_1-_1P1-O02_2, 2015
In this paper, to improve the spatiotemporal resolution and to reduce the invasiveness, we genetically introduce Optically-Manipulated molecular switch, channelrhodopsin (ChR), into sarcoplasmic reticulum (SR) of C2C12 myoblast, mouse muscle cell lines. A myotube inserted with SR-targeted ChR can respond rapidlier than a myotube inserted with non-targeted ChR for 10-20 ms. A myotube inserted with SR-targeted ChR can contraction only by intracellular ion transportation. We can control intracellular Ca^<2+> ion of the myotube. This technique could facilitate the studies on the myogenesis, the stem cell therapy for muscular disorder, and the bioengineering using the muscle-powered actuator.