20 0 0 0 OA 光学顕微鏡技術の過去・現在・未来
- 著者
- 佐甲 靖志
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.55, no.6, pp.317-321, 2015 (Released:2015-11-28)
- 参考文献数
- 31
The history of optical microscopy was started by van Leeuwenhoek, who discovered microorganisms for the first time in the world. Even at that time, the most essential powers of the optical microscopy were high spatial resolution, high sensitivity, and detection of motion. In this small review, we will recall how these three powers of optical microscopy have extended biophysical sciences. Detection and analysis of the individuality, distribution, dynamics, and history in the biological scales from single molecules to cells and tissues were the major roles of the optical microscopy. These roles inevitably lead optical microscopy to proceed toward the computational sciences.
16 0 0 0 OA Gタンパク質共役型受容体の生細胞1分子計測を薬効薬理評価に応用する
- 著者
- 柳川 正隆 佐甲 靖志
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.61, no.6, pp.366-369, 2021 (Released:2021-11-25)
- 参考文献数
- 20
G protein-coupled receptors (GPCRs) constitute one of the largest superfamily of proteins in the human genome. Since GPCRs are major drug targets, a pharmacological evaluation of compounds on a GPCR is an important process in drug discovery. Here, we review the past trends of the in cell single-molecule measurement of GPCRs, many of which were focused on dimerization of GPCRs. Then, we introduce our recent study regarding the drug evaluation based on a general feature of a diffusion-function relationship of GPCRs. We also discuss the future applicability of the present study in pharmacology and drug screening.
