10 0 0 0 OA 生物物理学の概念と展望
1 0 0 0 OA 整合性原理に基づくタンパク質デザイン
- 著者
- 古賀 理恵 古賀 信康
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.60, no.6, pp.325-330, 2020 (Released:2020-11-28)
- 参考文献数
- 26
Protein design holds promise for applications such as control of cells, therapeutics, new enzymes and protein-based materials. Recently, rational design of protein molecules has made a great progress, guided by the consistency principle proposed by Nobuhiro Gō in 1983: local and non-local interactions consistently favor the same folded conformation. We discovered a set of rules for designing ideal protein structures stabilized by consistent local and non-local interactions. The rules enabled the de novo design of amino acid sequences having the funnel-shaped energy landscapes toward the desired target structures. Various ideal protein structures have been created using the rules.
1 0 0 0 OA 理想タンパク質構造の設計原理
- 著者
- 古賀(巽) 理恵 古賀 信康
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.53, no.4, pp.190-193, 2013 (Released:2013-07-25)
- 参考文献数
- 15
- 被引用文献数
- 3
Protein folding occurs because the native interactions collectively outweigh non-native interactions, resulting in funnel-shaped energy landscapes. The funnel-shaped landscapes of natural proteins are rugged due to evolution for function or neutral drift. We describe an approach to designing ideal protein structures stabilized by consistent local and non-local interactions. The approach is based on rules relating local structures to tertiary motifs, which make possible the design of strongly funneled energy landscapes. Guided by these rules, we succeeded in designing five ideal alpha-beta protein structures with different topologies completely from scratch. These results illuminate how the folding funnels of natural proteins arise.