著者

冨岡 華代 北野 文理 北田 善三

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.22, no.2, pp.88-93, 2015

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Cyanogenic glycoside, amygdalin (AM) and its degradation products, benzoic acid (BA) and benzaldehyde (BAL) in rose family plants were measured utilizing high performance liquid chromatography (HPLC). Liqueur was cleaned in an InertSep NH₂ cartridge, and other samples were cleaned in an InertSep C18 cartridge and an InertSep NH₂ cartridge. The three components were not detected in any sarcocarp. AM was detected in seeds of loquat, apricot and cherry in the range of 1.35〜28.80 mg/g, and BAL was detected at 0.48 mg/g in seeds of apricot. AM and BA were detected at 0.83 mg/g and 0.10 mg/g in tea processed from loquat seed.

著者

冨岡 華代 北野 文理 北田 善三

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.22, no.2, pp.88-93, 2015-08-26 (Released:2017-01-27)

参考文献数

8

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Cyanogenic glycoside, amygdalin (AM) and its degradation products, benzoic acid (BA) and benzaldehyde (BAL) in rose family plants were measured utilizing high performance liquid chromatography (HPLC). Liqueur was cleaned in an InertSep NH2 cartridge, and other samples were cleaned in an InertSep C18 cartridge and an InertSep NH2 cartridge. The three components were not detected in any sarcocarp. AM was detected in seeds of loquat, apricot and cherry in the range of 1.35〜28.80 mg/g, and BAL was detected at 0.48 mg/g in seeds of apricot. AM and BA were detected at 0.83 mg/g and 0.10 mg/g in tea processed from loquat seed.

著者

冨岡 華代 高津 万結香 巽 美智 長谷川 絢 北田 善三

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.21, no.1, pp.15-20, 2014

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A method has been developed for determining amygdalin (AM) and its degradation products, benzyl alcohol (BeOH), benzaldehyde (BAL) and benzoic acid (BA) in Chinese quince fruit utilizing high performance liquid chromatography (HPLC). A sample was extracted with 0.05 mol/L citric acid solution (pH2.2) and cleaned in an InertSep C18 cartridge and an InertSep NH₂ cartridge. The HPLC separation was performed on an Inertsil ODS-4 column using acetonitrile-0.01 mol/L phosphate buffer (pH2.0) (15:85) in the mobile phase. A UV monitor was used for detection. The effects during growth period on the amount of 4 components of sarcocarp and seed of Chinese quince was examined. AM content in seed increased proportionally each month. This increase was especially significant in seed retrieved in November. AM was detected in commercially available Chinese quince in the range of 0.03-0.06 mg/g sarcocarp, 0.21-1.75 mg/g seed. BA was not detected in any samples.

著者

北田 善三 坊木 佳人

出版者

日本食品衛生学会

雑誌

食品衛生学雑誌 (ISSN:00156426)

巻号頁・発行日

vol.38, no.4, pp.J-241-J-244, 1997-08-05

著者

木口 智明 濱川 恵梨香 冨岡 華代 新居 朋恵 前田 雅子 堀江 正一 北田 善三

出版者

日本食品化学学会

雑誌

日本食品化学学会誌 (ISSN:13412094)

巻号頁・発行日

vol.17, no.2, pp.130-135, 2010

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A simple and rapid method has been developed for determining of L,L-aspartame (APM), its epimer (L,D-APM), diketopiperazine (DKP), L-phenylalanine (Phe) and D-Phe in various foods by high performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS). These compounds in foods were extracted with 0.01mol/L hydrochloric acid in ultrasonic bath, and the extract was loaded onto a strata-X-C, cation-exchange and reverse-phase cartridge. The HPLC separation was performed on a SUMICHIRAL OA-5000 (monolithic-type, 4.6mm i.d.×10cm) with ultraviolet detection, using 2mmol/L CuSO₄ solution-acetonitrile-isopropyl alcohol (85:10:5) as a mobile phase. The LC/MS separation was performed on a CHIROBIOTIC TAG (2.1mm i.d.×25cm, 5μm) with a mobile phase of 0.01% ammonium acetate-0.005% acetic acid-ethanol (1:1:2). The recoveries of 5 compounds from foods added at the level of 0.2g/kg in HPLC and LC/MS were 93.9-102.9% and 82.1-102.4%, respectively. In HPLC, the detection limits of APM, DKP and Phe were 0.02g/kg. In LC/MS, the detection limits of APM was 0.01g/kg, and DKP and Phe were 0.02g/kg.