著者

宮崎 勝己 小林 豊 鳥羽 光晴 土屋 仁

出版者

日本動物分類学会

雑誌

タクサ:日本動物分類学会誌 (ISSN:13422367)

巻号頁・発行日

vol.28, pp.45-54, 2010-02-20 (Released:2018-03-30)

参考文献数

44

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The biology of Nymphonella tapetis Ohshima, 1927, a pycnogonid endoparasitic on some bivalves, is reviewed. After the first discovery of this species from Hakata Bay, Fukuoka in 1926, there were scattered records of N. tapetis from several places in Japan before 2007, but they were on a small scale. In April 2007, N. tapetis appeared suddenly in the commercial bivalve, Ruditapes philippinarum and several other bivalves on the Banzu Tidal Flat in Tokyo Bay. The spread of the parasite was explosive, and caused a mass mortality of the bivalves in the area. Adults of the pycnogonid live freely on or just under the surface of sandy bottoms, and show nocturnal activity. The hatching larva is a typical protonymphon larva. The larva enters the host bivalve, attaches to various soft parts, and feeds on the body fluid of the host. The number of parasites in one host ranges from one to over 60. At least eight different developmental stages can be distinguished in the parasitic larvae and juveniles. Adults leave the host probably just after the maturation molt. The adult male receives one egg-mass onto his ovigers after each mating, and one male can bear up to seven egg-masses. Several experiments were undertaken to attempt to eradicate or reduce the number of N. tapetis, but no effective method has yet been found. Three species of the genus Nymphonella have been described from Japan, the Mediterranean, and southern Africa. They are very similar in morphology, which leads to potential taxonomic confusion.

著者

土屋 仁美

出版者

法政大学 (Hosei University)

巻号頁・発行日

pp.1-326, 2014-09-15

著者

近宗 雅人 猪之鼻 修一 横関 俊昭 土屋 仁 藤田 和弘

出版者

公益社団法人 日本食品衛生学会

雑誌

食品衛生学雑誌 (ISSN:00156426)

巻号頁・発行日

vol.59, no.5, pp.248-256, 2018-10-25 (Released:2018-11-14)

参考文献数

18

被引用文献数

1

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LC-MSおよびLC-MS/MSによるアクリルアミドの前駆体である遊離アスパラギン(Asn)の穀類中の分析法を開発した.試料中から5%(w/v)トリクロロ酢酸溶液でAsnを抽出し,0.1%(v/v)ギ酸溶液で適宜希釈した後,LC-MSまたはLC-MS/MSで測定した.分析カラムはPenta Fluoro Phenyl(PFP)カラムを用い,0.1%(v/v)ギ酸溶液とアセトニトリルのアイソクラティック溶出により行った.検量線は,0.005~0.1 μg/mLの範囲で良好な直線性を示した.片栗粉,うるち米粉,小麦全粒粉の3試料を用いて添加回収実験を行った結果,平均回収率は95.4~100.9%,併行精度(RSDr)は0.9~6.0%,室内精度(RSDwr)は2.8~7.1%の良好な結果が得られた.本法による定量限界は,片栗粉で7 mg/kg,うるち米粉で5 mg/kgであった.また,遊離アスパラギンを含有しているうるち玄米粉,コーンフラワー,小麦強力粉,小麦全粒粉,ライ麦粉の5種類を用いて10試験室による試験室間共同試験を実施したところ,HORRATr値は0.4~1.0とCodex委員会が定める性能基準の2以下であったことから,本法の有効性が示唆された.

著者

横関 俊昭 土屋 仁 藤田 和弘

出版者

公益社団法人 日本食品衛生学会

雑誌

食品衛生学雑誌 (ISSN:00156426)

巻号頁・発行日

vol.59, no.4, pp.187-191, 2018-08-25 (Released:2018-08-30)

参考文献数

14

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穀類中に存在するアクリルアミドの前駆体である遊離アスパラギンをダンシル誘導体化後,HPLC-UVで測定する方法を評価するため,9試験室による試験室間共同試験を行った.試料には,遊離アスパラギンを含有しているうるち玄米粉,コーンフラワー,小麦強力粉,小麦全粒粉,ライ麦粉の5種類を用い,各試験室2回の併行測定とした.試験の結果,併行相対標準偏差および室間相対標準偏差はそれぞれ,0.5~2.2%および2.3~5.9%であり,HorRat値は0.4~0.6とCodex委員会が定める性能基準の2以下であったことから,本法の有効性が示唆された.

著者

宮崎 勝己 小林 豊 鳥羽 光晴 土屋 仁

出版者

日本動物分類学会

雑誌

タクサ : 日本動物分類学会誌 (ISSN:13422367)

巻号頁・発行日

no.28, pp.45-54, 2010-02-20

被引用文献数

3

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The biology of Nymphonella tapetis Ohshima, 1927, a pycnogonid endoparasitic on some bivalves, is reviewed. After the first discovery of this species from Hakata Bay, Fukuoka in 1926, there were scattered records of N. tapetis from several places in Japan before 2007, but they were on a small scale. In April 2007, N. tapetis appeared suddenly in the commercial bivalve, Ruditapes philippinarum and several other bivalves on the Banzu Tidal Flat in Tokyo Bay. The spread of the parasite was explosive, and caused a mass mortality of the bivalves in the area. Adults of the pycnogonid live freely on or just under the surface of sandy bottoms, and show nocturnal activity. The hatching larva is a typical protonymphon larva. The larva enters the host bivalve, attaches to various soft parts, and feeds on the body fluid of the host. The number of parasites in one host ranges from one to over 60. At least eight different developmental stages can be distinguished in the parasitic larvae and juveniles. Adults leave the host probably just after the maturation molt. The adult male receives one egg-mass onto his ovigers after each mating, and one male can bear up to seven egg-masses. Several experiments were undertaken to attempt to eradicate or reduce the number of N. tapetis, but no effective method has yet been found. Three species of the genus Nymphonella have been described from Japan, the Mediterranean, and southern Africa. They are very similar in morphology, which leads to potential taxonomic confusion.