著者

大串 勉

出版者

特定非営利活動法人日本歯周病学会

雑誌

日本歯周病学会会誌 (ISSN:03850110)

巻号頁・発行日

vol.22, no.3, pp.307-318, 1980

被引用文献数

1

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The purpose of this investigation was to find out the relationship between volatile sulphur compounds in incubated whole saliva and that in mouth air, and between the former and cellular elements in whole saliva in periodontal patients.<br>The subjects were divided into the following three groups based on clinical findings and intensity of odour measured by olfactory panel of trained judges; the periodontal patients with halitosis (P-H group), the periodontal patients without halitosis (P-N group) and the normal subjects (N group)<br>The test samples used this investigation were unstimulated whole saliva and that were incubated at 37&deg;C.<br>The amount of valatile sulphur compounds produced from incubated whole saliva and that in mouth air were measured by gas chromatograph equipped with flame photometric detector, while the number of leucocytes and epithelial cells in whole saliva were counted by Klinkhamer's technique.<br>The degradation of leucocytes and epithelial cells in incubated whole saliva were observed using smear speciments staind with hematoxylin and eosin.<br>Results were as follows<br>1. The average number of leucocytes in whole saliva were 11764/cmm in P-H group, 3085/cmm in P-N group and 434/cmm in N group. Obvious differences were observed among three groups. The average number of epithelial cells in whole saliva were 1381/cmm in P-H group, 1157/cmm in P-N group and 310/cmm in N group. There were no differences between P-H group and P-N group.<br>2. The methyl mercaptan produced from incubated whole saliva in P-H group was detected earlier in time and largier in quantity than other two groups.<br>3. In P-H group, obvious correlation was observed between number of leucocytes in whole saliva and methyl mercaptan contents in mouth air, however, no correlation was observed between number of epithelial cells in whole saliva and methyl mercaptan contents in mouth air. In all subjects, obvious correlation was observed between number of leucocytes and the highest value of methyl mercaptan contents produced incubated whole saliva, however, no correlation was observed between number of epithelial cells and the highest value of methyl mercaptan contents produced incubated whole saliva.<br>4. Number of leucocytes in whole saliva were observed high correlation with gingival score, bleeding, discharge of pus and pocket depth, but no correlation between plaque score.<br>5. Leucocytes in incubated whole saliva degraded rapid and they were almostly not observed in 4 hours after incubation, however, degradation of epithelial cells were observed about 8 hours after incubation.

著者

角田 正健 佐藤 春海 大串 勉

出版者

特定非営利活動法人 日本歯周病学会

雑誌

日本歯周病学会会誌 (ISSN:03850110)

巻号頁・発行日

vol.23, no.3, pp.490-498, 1981-09-28 (Released:2010-07-16)

参考文献数

21

被引用文献数

9 18

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The purpose of this study was to clarify the effect of sodium copper chlorophyllin (Chn.) for halitosis.Volatile sulphur compounds (V. S. C) produced during the incubation of whole saliva were analyzed by gas chromatograph. The changing of leucocytes and epithelial cells in incubated whols saliva were observed using microscope. The effect of anti-bacteria against oral anaerobic bacteria was also observed.The following conclusions were obtained as the result of judging the halitosis control effect of Chn. by its experimental use of the saliva of periodontal patients having halitosis1. By adding and incubating 100mg, 50mg, 10mg and 5mg Chn. respectively into 1ml saliva, V. S. C. were not detected. However, when sampled at 1mg addition under the condition of incubate 36 and 48 hours, a small amount of V. S. C. was detected.2. The destruction speed of cellular components by incubate, in comparision with a controlled group, was not effected by addition of Chn.3. By microscopic observation, cell attachment on obsorption of Chn. were found which suggested its combination with protein.4. Almost any effect of anti-bacteria or bacteria growth prevention against oral anaerobic bacteria of Chn. was not observed.

著者

中川 種昭 磯部 秀一 池上 暁子 林 真理 渋川 義宏 住井 浩剛 高橋 潤一 轟 啓造 山田 真美 大串 勉 佐和 正彦 角田 正健 山田 了

出版者

特定非営利活動法人日本歯周病学会

雑誌

日本歯周病学会会誌 (ISSN:03850110)

巻号頁・発行日

vol.35, no.4, pp.674-680, 1993-12-28

被引用文献数

13 4

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2種類の電動歯ブラシと手用歯ブラシを用い,任意刷掃時間と3分におけるプラーク除去効果および手用歯ブラシの刷掃技術レベルの違いによる電動歯ブラシの刷掃効果について18名の被検者を用い検討した。その結果,電動歯ブラシにおける任意刷掃時間は,毛束反復回転式で平均7分3秒,ユニット運動式で7分22秒で,従来規定することの多い3分より長い時間であった。そのプラーク除去効果は3分と比較して10〜15%高く,その差は多くの被検者で舌側,隣接面といったプラークコントロールに重要な部位に認められる差であり,電動歯ブラシにおいてもある程度長い刷掃時間が必要であることが示された。また,手用歯ブラシの刷掃技術レベルの高い群と低い群での電動歯ブラシのプラーク除去効果について調べたところ,その刷掃効果は同程度であり,手用歯ブラシの刷掃技術が低い患者に対して電動歯ブラシの使用がとくに有効であることが示唆された。