3 0 0 0 OA タンパク質の反応と揺らぎ
- 著者
- 黒井 邦巧 寺嶋 正秀
- 出版者
- 一般社団法人 日本生物物理学会
- 雑誌
- 生物物理 (ISSN:05824052)
- 巻号頁・発行日
- vol.55, no.5, pp.235-241, 2015 (Released:2015-09-29)
- 参考文献数
- 28
Biological molecules fluctuate at room temperature in solution, because of the thermal motion. A relationship between fluctuations and biological reactions should be clarified for understanding molecular mechanism of biological reactions. Some methods to detect protein fluctuations are reviewed. In particular, a recently developed time-resolved method to measure the fluctuations based on the transient grating is described. This method was applied to a reaction of a blue-light sensor protein TePixD. This result clearly demonstrated that the compressibility, which reflects the fluctuation is enhanced during the reaction. It was also found that this enhanced fluctuation is a trigger of the reaction, supporting the importance of fluctuations for the biological function.
1 0 0 0 OA 新規な光トリガーを用いた蛋白質の高次構造変化ダイナミクス検出法の開発と応用
1 0 0 0 OA 蛋白質反応における体積変化と圧力効果
- 著者
- 寺嶋 正秀 木村 佳文 星原 悠司
- 出版者
- 日本高圧力学会
- 雑誌
- 高圧力の科学と技術 (ISSN:0917639X)
- 巻号頁・発行日
- vol.17, no.1, pp.4-12, 2007 (Released:2007-04-12)
- 参考文献数
- 29
In this article, reaction volumes and pressure effects on protein reactions are discussed by means of the pulsed laser induced transient grating (TG) spectroscopy. By the TG method, temporal profile of the protein reaction volume has been determined for the non-reversible reaction process. For examples, TG studies on the photo-chemical process of carboxymyoglobin and photoactive yellow protein are introduced. The reaction volume for each process has been determined, which can not be studied by the other spectroscopic methods such as transient absorption method. A high pressure optical system, which enabled us to measure the pressure dependence of the reaction volume, has also been reported.