著者

松木 光雄 渡部 俊彦 小笠原 綾子 三上 健 松本 達二

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.128, no.8, pp.1203-1207, 2008 (Released:2008-08-01)

参考文献数

10

被引用文献数

12 17

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Glutathione dose dependently inhibited melanin synthesis in the reaction of tyrosinase and L-DOPA. The inhibition of melanin synthesis was recovered by increasing the concentration of L-DOPA, but not recovered by increasing tyrosinase. Glutathione inhibited the binding between tyrosinase and L-DOPA. Although the synthesized melanin was aggregated within 1 h, the aggregation was inhibited by the addition of glutathione. These results indicate that glutathione inhibits the synthesis and agglutination of melanin by interrupting the function of L-DOPA.

著者

島崎 伸子 山森 徹雄 小笠原 綾子 川田 毅 沖野 憲司 塩山 司 石橋 寛二 佐藤 匡

出版者

Japanese Society of Psychosomatic Dentistry

雑誌

日本歯科心身医学会雑誌 (ISSN:09136681)

巻号頁・発行日

vol.8, no.1, pp.37-44, 1993-06-25 (Released:2011-09-20)

参考文献数

15

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Recently, many cases in which stress is the main cause of craniomandibular disorder (CMD) have been reported. Thus, in order to properly diagnose and treat the different stages of CMD, it becomes vitally important to objectively grasp the effect of stress.This report studied the changes in salivary pH of 15 normal adults with no CMD or autonomic nervous system (ANS) abnormalities. Stress was applied experimentally by having the subject perform and answer simple factorial equations under conditions of heavy lighting and sound for a period of 1 minute. Mixed resting saliva was collected before and after such stress was applied, and additional physiological parameters such as the pneumogram, electrocardiogram, and plethysmogram were also recorded to determine the state of the ANS. The saliva samples were obtained by sampling paper during the resting period before stress, immediately after stress, as well as at the 3, 6 and 10 minute marks. Furthermore, the saliva measurements were taken 9 minutes more for each time frame. The samples were covered with a plastic plate and the pH was measured. Measurements showed the pH value to stabilize after 1 minute. However, after removing the plastic plate and allowing volatization of CO2 gas, the pH began to elevate until it reached a maximum peak at approximately 5 minutes. This pH difference from the stable state was referred to as DIAL. Analyses concerning ANS were also recorded for 1 minute before collecting the saliva.In conclusion, the coefficiency of the plethysmogram amplitude increased due to slight stress, while stable pH ualues and DpH1 decreased after slight stress, thus indicating a relationship between the activity of the parameters involving autonomic responses and pH curve of the saliva. We concluded that these studies showed that stable pH values and DpH1 reflect the activity of the autonomic nerve responses.