著者

中林 亮 浅野 孝 山崎 真巳 斉藤 和季

出版者

公益社団法人 日本農芸化学会

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.52, no.5, pp.313-320, 2014-05-01 (Released:2015-05-01)

参考文献数

23

著者

山崎 真巳

出版者

公益社団法人 日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.55, no.7, pp.671-673, 2019 (Released:2019-07-01)

参考文献数

8

---

最近急速に進んだゲノム科学技術の発展により、植物における有用アルカロイド生合成についても、その特異な物質代謝がどのように進化してきたかに関するゲノムレベルでの解明が進んでいる。筆者らが明らかにしてきたアルカロイド生産の方向決定ステップを触媒する酵素の分子進化、ならびに毒性アルカロイドを生産する植物の自己耐性の分子進化について紹介し、今後の新しい生合成のデザインを目指した展開について説明する。

著者

山崎 真巳

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.122, no.1, pp.47-56, 2002-01-01 (Released:2003-02-13)

参考文献数

36

被引用文献数

2 2

---

A molecular biological approach was applied to the study of diversity and regulation of secondary metabolism in medicinal plants at various levels. For the inter-species diversity, RFLP (restriction fragment length polymorphysm) and RAPD (random amplified polymorphic DNA) analyses of genomic DNA were performed on the plants, belonging to the same genus or family and containing related compounds. Phylogenetic trees of lupin alkaloid containing plants and other medicinal plants, based on RFLP and/or RAPD profiles, showed the relationship between the diversities in genomes and secondary metabolisms. The chemotypes regarding anthocyanin production in Perilla frutescens var. crispa, were subjected to the study on intra-species diversity. The structural genes and the regulatory genes involved in anthocyanin biosynthesis were isolated and their expression in red and green forms was determined by Northern blot analysis. The expression of all structural genes examined was co-ordinately regulated in form-specific manner and by light illumination. The anthocyanin production was enhanced in transgenic plants over-expressing Myc homologue genes from perilla. These results suggested that a protein complex including bHLH factors might regulate the expression of a series of structural genes. Additionally, cDNAs coding anthocyanin 5-O-glucosyltransferase and anthocyanidin synthase were isolated and characterized using recombinant proteins for the first time. In conclusion, it was indicated that the molecular biological techniques are powerful tools for the investigation of diversity and regulation of and for the genetic engineering of secondary metabolism in medicinal plants.