著者

山縣 邦弘 小山 哲夫 小林 正貴 成田 光陽

出版者

Japanese Society of Nephrology

雑誌

日本腎臓学会誌 (ISSN:03852385)

巻号頁・発行日

vol.32, no.10, pp.1045-1052, 1990

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This study was undertaken to analyze the mechanisms of immune complex formation in Heymann nephritis. We isolated two different RTE antigens by gel filtration and prepared rabbit antisera against these antigens. By the indirect immunoflurescence method using normal rat renal tissues, the 65, 000 molecular weight antigen (=β) was observed not only in RTE, but also in the glomerular epithelium, epithelium of the small intestine, liver and spleen. On the other hand, the 35, 000 molecular weight antigen (=γ) existed in RTE and epithelium of the small intestine. When rats were injected intravenously with rabbit antiserum against β, glomerular depositions were observed within two hours. In rats injected with rabbit antiserum against r, no glomerular deposition was seen with-in 2 days, but fine granular depositions were observed after 6 days. When rat kidneys were perfused with rabbit antiserum against γ in saline by a single pass method, no glomerular deposition was seen. However, in rat kidneys perfused with preformed soluble γ-anti γ IC, fine granular depositions along the capillary walls were seen soon after the perfusion. Further the antigen which was reacted with anti γ antiserum was isolated from normal rat serum by immuno-affinity chromatography. These facts suggest that the mechanisms of IC formation may be due to not only in situ immune complex formation but also circulating immune complex deposition in Heymann nephritis.

著者

石田 久美子 石田 裕 山縣 邦弘 小山 哲夫 成田 光陽

出版者

一般社団法人 日本内科学会

雑誌

日本内科学会雑誌 (ISSN:00215384)

巻号頁・発行日

vol.90, no.7, pp.1199-1206, 2001-07-10 (Released:2008-06-12)

参考文献数

10

被引用文献数

1

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成人では職域,地域で健診が実施されているが,学童と異なり,暫定診断,結果の集計まで含めたシステム化は行われていない.茨城県の健診結果では,血清クレアチニン(S-Cr)異常者は尿蛋白陽性者に多く,また,年代が高いほど多い.一方, S-Cr異常者の6割以上が尿検査で異常を認めない.腎機能予後は尿蛋白陽性群,高血圧群で悪い.軽度腎機能異常者は腎障害としての治療を受けていない者が多く,成人領域における管理システムの構築が望まれる.

著者

竹村 克己 青柳 一正 永瀬 宗重 坂本 まさ子 石川 敏子 成田 光陽

出版者

社団法人 日本腎臓学会

雑誌

日本腎臓学会誌 (ISSN:03852385)

巻号頁・発行日

vol.32, no.11, pp.1195-1201, 1990 (Released:2011-03-01)

参考文献数

19

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Methylguanidine (MG) which is known as a uremic toxin, is synthesized from creatinine(Cre). We have clarified that active oxygen plays an important role on MG synthesisin vitro and in rat hepatocytes. On the other hand, hyperoxia is very injurious in various tissues, and it has been reported that active oxygen produced in hyperoxia plays an important role on the tissue injury. This study was performed to investigate the effect of hyperoxia on MG synthesis in vivo. The subjects in this study were patients who were treated by hyperbaric oxygen therapy (HBO), Serum Cre, MG, and urinary Cre, MG before and after HBO were measured in these subjects. The subjects were classified into four groups. Group 1-III were undergone HBO with condition of 100% 02, 2 atomosphere absolute (ATA), 1 hour, (I: Ccr<10 ml/min, II: l0≤Ccr<50 m1/min, III: Ccr≥50ml/min) and group IV (Ccr≥50ml/min) with 100%O2, 3ATA. 1 hour. Urinary excretion rate of MG (urine MG/urine Cre) significantly increased after HBO therapy in every group. Urine MG/urine Cre/serum Cre ratio which was used as a index of MG synthesis rate also increased. In this study, it is clarified that MG excretion rate increases in hyperoxic condition. These results suggest that active oxygen plays an important role on MG synthesis in vivo, and that the urine MG/urine Cre/serum Cre ratio can be a uaeful maker of the active oxygen products in vivo.