著者

斎藤 肇 佐藤 勝昌 冨岡 治明 井上 圭太郎 重藤 えり子

出版者

JAPANESE SOCIETY FOR TUBERCULOSIS

雑誌

結核 (ISSN:00229776)

巻号頁・発行日

vol.67, no.2, pp.89-95, 1992-02-15 (Released:2011-05-24)

参考文献数

18

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Fourty-five sputum specimens collected at the National Sanatorium Hiroshima Hospital were subjected to cultivation using either BACTEC 460 TB System (BACTEC method; Becton Dickinson Co., Towson, Md., U.S.A.) or 3% Ogawa egg medium. Test suptum was treated with four volumes of 4% NaOH for approximately two minutes, after which 0.1ml of the treated sputum was immediately inoculated onto 3% Ogawa egg medium. After neutralizing the remaining pretreated sputum with 1N HCl, and diluting with 1/15 M phosphate buffer PB; pH 6.8), it was then centrifuged at 3, 000rpm for 20min and the sediment was suspended in 1.5 ml of PB. Volumes of 0.5 ml each were inoculated into BACTEC 12B medium (4ml), containing PANTA for prevention of contamination and POES for promoting the growth of mycobacteria.In the BBCTEC method, bacterial growth was measured in terms of increases in the Growth Index (GI) values which were determined by the amount of 14CO2 released from the 14C -labelled palmitate during cultivation at 37°C (positive growth;GI≤50). Moreover, ρInitro-α-acetylamino-β-hydroxy-propiophenone (NAP)-sensitivity testing was done by transferring a part of the BACTEC 12B culture showing positive growth to a NAP vial, and thereafter subjected to further cultivation.Among the 45 sputum specimens, the number of positive specimens for mycobacterial growth in the afore mentioned cultivation methods and time required for growth were as follows: 3% Ogawa egg medium, 12 specimens (27%), seven M. tuberculosis complex strains at 12-35 days (average 21 days), five M. avium complex strains at 14-21 days (average 18 days): BACTEC method, 18 specimens (40%), 11 M. tuberculosis complex strains at 3-28 days (average 14 days), six M. avium complex strains at 3-10 days (average 6 days) and one M. scrofulaceum strain at 28 days. There were no specimens that tested positive for mycobacterial growth on 3% Ogawa egg medium but negative in BACTEC 12B medium. The BACTEC method was most efficacious in cultivating acid-fast bacilli from smear-negativeWhen NAP-sensitivity testing was done using the BACTEC method, mycobacteria in 11 test sputa were deteremined as NAP-sensitive, thereby belonging to M. tuberculosis complex. The fact that all of the organisms determined as NAP-sensitive using the BACTEC method were rough and nonphotochromogenic, and identified as M. tuberculosis complex by AccuProbeTM testing, confirmed the reliability of NAP-testing. sputa.The mycobacteria in seven sputum specimens detected using the BACTEC method were determined as NAP-resistant. Six of them were smooth and nonphotochromogenic, and identified as M. avium complex by AccuProbe testing. The one remaining strain was a scotochromogen with a smooth colony morphology, and had no reaction to either the M. tuberculosis complex- or M. avium complex-AccuProbe tests. This strain was identified as M. scrofulaceum using an α-antigen analysis.These results indicate the usefulness of the BACTEC 460 TB system in the rapid diagnosis of mycobacteria including M. tuberculosis complex and M. avium complex.

著者

青木 正和 片山 透 山岸 文雄 横田 総一郎 亀田 和彦 斎藤 肇 原 耕平 江崎 孝行 河合 忠 四元 秀毅 関口 進

出版者

一般社団法人 日本結核病学会

雑誌

結核 (ISSN:00229776)

巻号頁・発行日

vol.69, no.10, pp.593-605, 1994-10-15 (Released:2011-05-24)

参考文献数

8

被引用文献数

8

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Recently, a new kit to detect and indentify mycobacteria in clinical specimens was developed by Japan Roche Co. Limited. The new method is based on amplification of DNA of mycobacteria in clinical specimens by PCR and hybridization of amplified DNA b. microwell plate hybridization method, which is the “AmplicorTM Mycobacteria, Roche. (AMP-M) ”. Cooperative study was organized with 15 tuberculosis hospitals and institu tions throughout Japan, and 349 clinical specimens from newly admitted tuberculosis patients and/or suspects were collected during July and August, 1993. All the specimens were examined by smear microscopy (Ziehl-Neelsen's staining), culture on Ogawa egg media, culture on variant 7H9 liquid media and by AMP-M. Excluding 25 specimens which had failed to identify the species of mycobacteria because of contamination, disability to multiply on the transplanted solid media and so on, the results of the examinations in 324 specimens consisting of 167 specimens from previously untreated cases and those of 157 specimens from previously treated cases were analysed. Main results obtained were as follows;1. Of 70 smear positive specimens from previously untreated cases, culture positive on Ogawa media and 7H9 media, and by AMP-M positive were 59 (84.3%), 61 (87.1%) and 66 (94.3%), respectively. Of 97 smear negative specimens, culture positive were 20 (20.6%), 22 (22.7%) and 27 (27.8%), respectively. The AMP-M showed the highest positive rate in both groups.2. The sensitivity and the specificity of AMP-M in previously untreated cases were calculated by assuming that positive on Ogawa and/or variant 7H9 media is “positive”. The sensitivity was 95.8% (68/71) and the specificity was 94.8% (91/96) for M. tuberculosis in previously untreated cases. The sensitivity and the specificity for M. avium and M. intracellulare were all 100%, although the numbers observed were small.3. So-called false positive of the AMP-M were observed in 5 cases out of 96 culture negatives on both Ogawa and variant 7H9 media. However, all 5 cases were positive by repeated AMP-M, 3 become culture positive later, and another 2 showed clinical findings consistent with tuberculosis. Hence, the authors considered that the false positive rate of the AMP-M method is to be very low in previously untreated cases.4. Of 86 smear positive cases with history of previous chemotherapy, the positive culture on Ogawa media, variant 7H9 media and that by AMP-M method were 64 (74.4%), 77 (89.5%) and 85 (98.8%), respectively. In the smear negative cases, culture positive was 10 out of 71 (14.1%), 13 (18.3%) and 24 (33.8%), respectively.5. The sensitivity and the specificity of the AMP-M were 98.7% (77/78) and 81.0% (64/79) for M. tuberculosis in previously treated cases calculated by the same method as in previously untreated cases. They were 77.8% (7/9) and 100% (148/148) for M. avium, and 100% (4/4) and 100% (153/153) for M. intracellulare.Based on these results, the authors concluded that the AMP-M is a very efficient and rapid method to detect and identify M. tuberculosis, M. avium and/or M. intracellulare in clinical specimens. This method will be useful to diagnose tuberculosis and diseases caused by mycobacteria other than M. tuberculosis rapidly.

著者

鈴木 久男 太田 和秀 斎藤 肇

出版者

公益社団法人日本セラミックス協会

雑誌

窯業協會誌 (ISSN:18842127)

巻号頁・発行日

vol.95, no.1098, pp.170-175, 1987-02-01

被引用文献数

9 18

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Densification behavior of powder compacts having a cordierite composition prepared by the sol-gel process using metal alkoxides is studied. The calcination of the alkoxy-derived powder at 800°C for 12h gave the best sintering. Densification of the powder compacts occurred from 800° to 900°C. Dense cordierite ceramics with different crystalline phases or properties were obtained by sintering the powder compacts without any sintering aid. The sintering of the powder compact at 1300°C for 12h gave dense α-cordierite ceramics having the flexural strength of 120MPa and the critical stress intensity factor of 2.8MN/m^3/2.

著者

斎藤 肇 螺良 英郎 山中 正彰 青柳 昭雄 大角 光彦 阿部 千代治 小倉 剛 前倉 亮治 中川 勝 河合 忠 久世 文幸 鈴木 克洋 坂谷 光則 高嶋 哲也 森 三樹雄

雑誌

臨床と微生物 = Clinical microbiology (ISSN:09107029)

巻号頁・発行日

vol.24, no.6, pp.897-903, 1997-11-25

被引用文献数

22

著者

山本 正彦 荒井 秀夫 河原 伸 岸 不盡弥 倉島 篤行 近藤 有好 坂谷 光則 佐藤 滋樹 原 耕平 水谷 清二 一山 智 喜多 舒彦 久世 文幸 斎藤 肇 下出 久雄

出版者

JAPANESE SOCIETY FOR TUBERCULOSIS

雑誌

結核 (ISSN:00229776)

巻号頁・発行日

vol.73, no.10, pp.599-605, 1998-10-15

被引用文献数

65

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日本結核病学会治療委員会は1987年に「非定型抗酸菌症の治療に関する見解」を発表したが, 本委員会は, 最近の10年間の非定型抗酸菌症に関する研究の進歩を踏まえて, 「非定型抗酸菌症の治療に関する見解-1998年」を公表することとした.