著者

河野 潤一 清水 晃 梅田 史郎 木村 重

出版者

神戸大学農学部

雑誌

神戸大学農学部研究報告 (ISSN:04522370)

巻号頁・発行日

vol.19, no.1, pp.p85-92, 1990

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日本産およびアメリカ産肝蛭を家兎に単数感染させ,各種変化について検索した。臨床症状は認められなかった。肝蛭卵および沈降抗体の検出時期は多数感染例と同様であった。肝病変は,肉眼的にも組織学的にも軽微であった。血液性状は,白血球数および好酸球数の若干の増加を認めた。血清の生化学的性状は,日本産肝蛭感染家兎において感染後9-10週に,GPT活性および総コレステロールの上昇を認めたが,そのほかでは著しい変化はなかった。肝蛭単数感染家兎の胆嚢から採集した虫卵の孵化率は,多数感染例におけるそれより低率であった。感染虫体の発育状況は,単数および多数感染例とも同様であった。 / Rabbits were experimentally infected either perorally with a single metacercaria or intraperitoneally with a newly excysted juvenile fluke of Japanese Fasciola sp. and American Fasciola hepatica. No clinical signs were noted. Fluke eggs were first detected in feces at the 63rd and 53rd post infection days for F. sp. and F. hepatica, respectively. Precipitating antibodies were first detected in sera at the 4th post infection week for both of the Fasciola species. Gross lesions showing adhesions, haemorrhages, nodules, scars, hyperplasia of connective tissue and thickening of the bile duct were all moderate. Histopathologically, tract lesions and haemorrhages were noted. In hematological examinations, no remarkable changes were observed in erythrocyte counts, leukocyte counts and hemoglobin content. Eosinophil percent increased in 6-8 weeks after infection. In biochemical examinations of sera, no remarkable changes were noted in concentrations of total protein, albumin and globulin, and the GOT activity. The GPT activity and total cholesterol concentration increased at the 10th and 9th post infection weeks, respectively, in the rabbits infected with F. sp. Hatching rates of fluke eggs that were collected from the gallbladder of the infected rabbits were 5-46% for F. sp. and 7-51% for F. hepatica. While, hatching rates of eggs that were collected from those infected with a multiple dose of Fasciola were 18-57% for F. sp. and 67-81% for F. hepatica. Body size and development of the inner organs of the flukes recovered from the rabbits that were infected with a single dose were the same as those recovered in the infections of multiple dose of the Fasciola species.