1 0 0 0 OA ダカルバジンの光分解に対する新規遮光カバーの有用性の検討
- 著者
- 森尾 佳代子 津金 麻実子 岡本 禎晃 糀 桂子 田墨 惠子 上島 悦子
- 出版者
- 一般社団法人日本医療薬学会
- 雑誌
- 医療薬学 (ISSN:1346342X)
- 巻号頁・発行日
- vol.39, no.6, pp.381-387, 2013-06-10 (Released:2014-06-10)
- 参考文献数
- 14
- 被引用文献数
- 2 2
Dacarbazine (DTIC) produces adverse reactions including local venous pain during the intravenous injection. DTIC is reported to be photolyzed to produce certain kinds of pain producing substances. 5-diazoimidazole-4-carboxamide (Diazo-IC) is considered to be a causative photolyte of venous pain. A newly designed cover shield has been used at Osaka University Hospital when DTIC is administered for the last 4 years. This shield comprises black cotton and covers both the infusion bag and route of infusion. We evaluated the effectiveness of this new shield against photodegradation of DTIC by determining the concentration of Diazo-IC. DTIC was dissolved with an injection solvent and mixed with 5% dextrose in water. Prepared samples were divided into 3 groups (without shield, infusion bag covered with shield, and infusion bag and infusion route covered with shield) and exposed under natural light conditions indoors. Prepared solutions ran down through the route and those samples were taken before and after passing the route pipe. Diazo-IC in the samples was measured by HPLC. Production of Diazo-IC in the non-covered bag was significantly increased in comparison with that in covered infusion bags. Diazo-IC production in samples after passing through the route was significantly increased compared with that in samples taken before passing through the route of the non-covered shield and covered infusion bag only. For the covered infusion bag and infusion route, the samples taken before and after passing through the route did not show significant differences. These data suggest that the new shield, which almost perfectly covers both the infusion bag and route of infusion, is effective in preventing DTIC photodegradation.
昨年度までに、リポソーム内でmiRNAを等温増幅し、検出することに成功した。これはプライマー、DNAポリメラーゼ、制限酵素を用いて標的microRNAを55℃の等温で増幅し、蛍光プローブ(SYBR GreenⅠ)で検出する方法である。本年度は等温増幅反応試薬内封リポソームとエキソソームと融合させ、リポソーム内でエキソソーム由来microRNAの検出を試みた。オクタデシルローダミンで膜染色したエキソソームと核酸等温増幅反応の反応液を内封したリポソームを混合後、電気刺激により膜融合させて55℃で2時間反応させた。しかし、リポソーム内でSYBR GreenⅠの蛍光は観察されなかった。電気融合はエレクトロポレーション用のキュベットを用いたが融合効率が低いことや、エキソソーム内のmiRNA濃度が低いことが理由として考えられる。最近、カバーガラスとアルミテープで作製した電気融合チャンバーを用いると融合効率が高く、さらに顕微鏡観察下で融合を実施できることがわかった。そこで、融合効率を上げるためのマイクロチャンバーデバイスの開発を行った。