著者

宮坂 祥夫 吉野 峰生 瀬田 季茂

出版者

バイオメカニズム学会

雑誌

バイオメカニズム (ISSN:13487116)

巻号頁・発行日

vol.12, pp.1-11, 1994-08-10 (Released:2016-12-05)

参考文献数

7

被引用文献数

1 1

著者

丸茂 義輝 瀬田 季茂

出版者

公益社団法人 日本薬学会

雑誌

衛生化学 (ISSN:0013273X)

巻号頁・発行日

vol.27, no.6, pp.381-387, 1981-12-31 (Released:2008-05-30)

参考文献数

20

被引用文献数

1 1

---

It has been noted that the contents of trace elements in hairs vary with difference of position in the same head, age, sex, hair cosmetic treatment and enviromental pollution. This paper describes the rapid analytical method of calcium in small hair segments (1 cm) using flameless atomic absorption spectrophotometry and the variation of calcium content in the hairs with the permanent wave treatment. Calcium was determined with the rocovery of 99% by ashing in platinum crucible followed by dissolving the residue in water by stirring with the glass covered magnetic spin bar. Loss of calcium was observed when the porcelain crucible or the glass flask was used as ashing ware, and the tefron covered magnetic spin bar caused contamination of calcium. The analytical results obtained by this method agreed with those of the other investigators determined by neutron activation analysis or atomic absorption analysis. In the hairs which had never permanent-waved the calcium concentrations were 200-1000 ppm and showed a general tendency to increase slightly from the root to the distal end of the hair. On the other hand, the permanent-waved hairs showed higher calcium concentrations (2000-4500 ppm). Adsorption of calcium on the treated hairs was dependent on pH, showing that after the hair previously treated with permanent wave reagents were soaked in city water (pH 6.8) calcium content increased and that no significant increase was observed by soaking in acidic calcium solution (pH 3). Calcium adsorbed on the treated hairs was removed by soaking in dilute hydrochloric acid (1→20). The above results suggested that permanent wave treatment is responsible for increasing ionexchangeable calcium in the hair.

著者

瀬田 季茂

出版者

日本法科学技術学会

雑誌

日本鑑識科学技術学会誌 (ISSN:13428713)

巻号頁・発行日

vol.1, no.1, pp.1-10, 1996 (Released:2010-06-10)

参考文献数

79

著者

関口 和正 今泉 和彦 藤井 宏治 千住 弘明 水野 なつ子 坂井 活子 笠井 賢太郎 佐藤 元 瀬田 季茂

出版者

日本法科学技術学会

雑誌

日本鑑識科学技術学会誌 (ISSN:13428713)

巻号頁・発行日

vol.2, no.1, pp.33-40, 1997 (Released:2010-02-06)

参考文献数

29

被引用文献数

11 11

---

Nucleotide sequences of 2 hypervariable regions (HV1, HV2) within the control region of human mitochondrial DNA (mtDNA) were analyzed from 55 unrelated Japanese. About 700 nucleotides were sequenced by using the nested PCR and the solid-phase direct sequencing methods. Comparison of these sequences with Anderson's reference sequence revealed 97 mutation types within 93 positions, and 11 positions of them were novel. Fifty five samples analyzed were classified into 52 different sequences, while 3 pairs have shown the same sequences. Comparison of the Japanese sequences to those reported from other populations indicated many differences in such a point that the substitutions at 16,223 and 73 in Japanese were more frequent than those in Caucasian, while the substitutions at 16,126 and 16,311 in Japanese were less frequent than those in Caucasian. Twenty one of 55 samples analyzed showed a T-to-C transition at the position 16,189 of the C-stretch region in the HV1 region. This replacement caused the blurred bands on the sequence image, which resulted in the ambiguity of exact number of cytosine in the C-stretch region of HV1. For this ambiguity, the number of cytosine in the C-stretch region should not be currently taken into account in forensic practices of individualization of evidence samples. Regardless of such problem, the polymorphisms of HV1 and HV2 regions are highly useful for individual identification.