著者

石原 研治 Hong JangJa Zee OkPyo 大内 和雄

出版者

公益社団法人 日本薬理学会

雑誌

日本薬理学雑誌 (ISSN:00155691)

巻号頁・発行日

vol.125, no.5, pp.265-270, 2005 (Released:2005-07-01)

参考文献数

48

被引用文献数

1

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気管支喘息などのアレルギー性疾患では,白血球の1つである好酸球が骨髄,末梢血および炎症部位で増加し,炎症部位に浸潤した好酸球は細胞内顆粒を放出することによって組織を傷害すると考えられてきた.しかし,最近,炎症部位に浸潤した好酸球は,炎症の誘発ではなく気道リモデリングに関与しているのではないかということが示唆され,病態形成における好酸球の役割に対する認識は変化しつつある.本稿では,最近の知見をふまえ,アレルギー性炎症における好酸球増多機構と炎症部位での好酸球の役割について述べる.

著者

石原 研治

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.125, no.9, pp.717-723, 2005-09-01 (Released:2005-09-01)

参考文献数

36

被引用文献数

2 2

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Eosinophils are one of the cells that play a critical role in the pathogenesis of allergic diseases. The increase in the number of eosinophils in such diseases is regulated by interleukin-5 (IL-5). The author have prepared recombinant rat IL-5 using a baculovirus expression system and examined its biological activities in rat eosinophils. It was demonstrated that recombinant rat IL-5 prolongs the survival of mature eosinophils and differentiates immature eosinophils into mature eosinophils, suggesting that rat IL-5 is a factor for eosinophilia in rats. Recombinant rat eosinophil-associated ribonuclease (Ear)-1 and Ear-2 were also prepared. Eosinophil granule proteins are thought to cause tissue damage due to their cytotoxic activity, but using recombinant rat Ear-1 and Ear-2, it was found that rat Ear-1 and Ear-2 have strong RNase A activity and bactericidal activity, suggesting that these proteins play critical roles in host defense. Finally, the important role of acetylation of histones was clarified in the differentiation of HL-60 clone 15 cells into eosinophils using the histone deacetylase inhibitors sodium n-butyrate, apicidin, and trichostatin A. These findings would be useful for further investigations of the role of eosinophils in allergic inflammation.