著者
吉田 渉 玉井 敦司 谷中 俊広 石田 幸子
出版者
弘前大学農学生命科学部
雑誌
弘前大学農学生命科学部学術報告 (ISSN:13448897)
巻号頁・発行日
no.4, pp.16-23, 2002-03-27

We indicated that the breeding season of Stichopus japonicus Selenka living in Mutsu Bay is from May to August based on the analysis of several reports. The adult sea cucumbers transferred from cool sea water (10℃) to warm sea water (20℃) started ejaculation and spawning within an hour. In the artificial induction, the best season may be during the end of May to the first ten days of June, because of the large number of mature eggs were collected from an adult sea cucumber. The number is about 3,000,000 eggs per individual. The normal development was as follows:(1)blastula:(13 hrs after fertilization), (2)gastrula:(19-30 hrs),(3)auricularia:(2-9 days),(4)doliolaria:(15-16 days),(5)pentactula:(18-22 days). When sea cucumber larvae were bred in the condition added Chaetoceros gracilis and commercial tropical fish food (TetraMin禾 baby) at low density (a larva / ml) in filtration sea water, they grew up into juvenile sea cucumbers. In this paper we reported the table of the stages in the normal development of sea cucumber, Stichopus japonicus Selenka under such conditions at 20℃.
著者
藤友 崇 森本 佳伸 大井 潤 澤井 典子 石田 幸子 松原 善 一圓 剛 皆川 直人 鴨井 久博 田中 司朗 鴨井 久一
出版者
特定非営利活動法人 日本歯周病学会
雑誌
日本歯周病学会会誌 (ISSN:03850110)
巻号頁・発行日
vol.60, no.2, pp.70-86, 2018-06-29 (Released:2018-06-29)
参考文献数
24

歯周病のリスク評価ができる自己申告アンケートは,歯周病の早期発見や疫学研究において有効な手段となるが,これまでに統計的,臨床的な妥当性を評価された日本人向けの歯周病リスク評価アンケートが開発された事例はない。本研究では,歯周病のリスクを評価できる日本語版自己申告アンケートを開発することを目的とした。歯周病患者50名と非歯周病患者51名に,歯周病で観察される症状の有無をアンケート形式で回答させた。アンケート回答に対して,多重ロジスティック回帰分析を実施し,歯周病を予測できるアンケート項目を抽出し,アンケートの質問項目の信頼性を確認した。また,ROC曲線解析を実施し,抽出されたアンケート項目による歯周病のリスク予測の精度を検討した。結果,50人の歯周病患者および50人の非歯周病患者を解析対象とした。多重ロジスティック回帰分析と歯周病の臨床的観点から,年齢,歯肉の腫れ,歯の動揺,プラークと歯石,口臭および掻痒感の6つのアンケート項目が自己申告によって歯周病のリスクを予測できる項目であることが分かった。また,ROC曲線解析によりAUCが0.90であった。本研究より,我々は,6つの自己申告アンケートで歯周病のリスクを判定できる日本人向けの歯周病セルフチェックアンケートを開発し,その信頼性,内部整合性及び精度を,40歳から83歳の大学病院歯科を受診した人を対象に検証した。
著者
秋山 朝子 今井 かおり 石田 幸子 伊藤 健司 小林 正志 中村 秀男 野瀬 和利 津田 孝雄
出版者
公益社団法人 日本分析化学会
雑誌
分析化学 (ISSN:05251931)
巻号頁・発行日
vol.55, no.10, pp.787-792, 2006 (Released:2006-11-17)
参考文献数
7
被引用文献数
2 3

An analytical method for the determination of aromatic compounds exhalated from hand skin has been proposed. The sampling of exhalated aromatic compounds was performed as follows: after the intake of aromatic compounds included in chewing gum or a capsule, exhalated skin gas was collected from a hand. The hand was covered with a sampling bag of poly vinyl fluoride (PVF) for 30 min. Then, the inner space of the sampling bag was sprayed with a 25% of ethanol aqueous solution. After removing the hand from the bag, the trapped solution containing skin gas was collected. The aromatic compounds in the trapped solution were extracted to the solid phase as Twister® (stir bar coated with poly dimethyl siloxane, Gerstel). Extracts were determined by gas-chromatograph mass spectrometry using a thermo desorption system and a selective ion mode. Linalool, citronellol and geraniol, which are the main components of rose essential oil, were detected from the skin of a hand after an oral intake of rose oil. The exhalated absolute amount of linalool, citronellol and geraniol increased in 30 to 60 min, and then decreased after intake. The recoveries of linalool, citronellol and geraniol were 53.5%, 66.7% and 55.1%, respectively. The correlation coefficient of the standard curves for linalool, citronellol and geraniol were 0.9977, 0.9994 and 0.9987, respectively. Each compound exahalated from the skin of a human body during 6 hours after intake was estimated to be, according to the amount of intake, 0.39%, 0.09% and 0.25%, respectively, for one subject. The absolute amount of geraniol exhalated from a hand increased significantly after oral intake for 8 subjects (P<0.025). This is the first report to present hard proof that an aromatic compound was exhalated from human skin after its intake as food.
著者
秋山 朝子 今井 かおり 石田 幸子 伊藤 健司 小林 正志 中村 秀男 野瀬 和利 津田 孝雄
出版者
公益社団法人日本分析化学会
雑誌
分析化学 (ISSN:05251931)
巻号頁・発行日
vol.55, no.10, pp.787-792, 2006-10-05
被引用文献数
1 3

An analytical method for the determination of aromatic compounds exhalated from hand skin has been proposed. The sampling of exhalated aromatic compounds was performed as follows: after the intake of aromatic compounds included in chewing gum or a capsule, exhalated skin gas was collected from a hand. The hand was covered with a sampling bag of poly vinyl fluoride (PVF) for 30min. Then, the inner space of the sampling bag was sprayed with a 25% of ethanol aqueous solution. After removing the hand from the bag, the trapped solution containing skin gas was collected. The aromatic compounds in the trapped solution were extracted to the solid phase as Twister^[○!R] (stir bar coated with poly dimethyl siloxane, Gerstel). Extracts were determined by gas-chromatograph mass spectrometry using a thermo desorption system and a selective ion mode, Linalool, citronellol and geraniol, which are the main components of rose essential oil, were detected from the skin of a hand after an oral intake of rose oil. The exhalated absolute amount of linalool, citronellol and geraniol increased in 30 to 60min, and then decreased after intake. The recoveries of linalool, citronellol and geraniol were 53.5%, 66.7% and 55.1%, respectively. The correlation coefficient of the standard curves for linalool, citronellol and geraniol were 0.9977, 0.9994 and 0.9987, respectively. Each compound exahalated from the skin of a human body during 6 hours after intake was estimated to be, according to the amount of intake, 0.39%, 0.09% and 0.25%, respectively, for one subject. The absolute amount of geraniol exhalated from a hand increased significantly after oral intake for 8 subjects (P<0.025). This is the first report to present hard proof that an aromatic compound was exhalated from human skin after its intake as food.