著者

山室 匡史 宮本 重彦 立入 直紀 石井 歩 松田 駿太朗 岩田 祐子 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.26, no.1, pp.29-48, 2021 (Released:2021-01-31)

参考文献数

31

被引用文献数

3 1

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The forensic identification of cannabis is performed by a combination of chemical analysis and morphological examination. Recently, molecular biological analysis using cannabis DNA information has been noticed as a new approach. In this study, the cannabis DNA detection kit using a DNA chromatography chip was developed, and the demonstration evaluation in the forensic chemical laboratory was carried out. The DNA detection kit of a “four-line version” which had the function to distinguish fiber-type from drug-type cannabis showed as high accuracy (98.3%) as the current identification method on cannabis identification. However, there was a tendency to mistake a part of the drug-type samples as “fiber-type cannabis”. In the kit of a “three-line version” which was specialized for the cannabis DNA detection, the accuracy of 99.0% was confirmed on the cannabis identification. There were no false positives throughout all evaluations. In addition, some of the combustion residues that could not be identified as cannabis by the current identification method were classified to be “cannabis positive” by the DNA detection kit, indicating the effectiveness of a new approach. As a result of this study, it was shown that the quick and accurate cannabis DNA analysis could be carried out by the DNA detection kit even by analytical chemists who didn't have expertise in molecular biology.

著者

立入 直紀

出版者

宮城県警察科学捜査研究所

雑誌

奨励研究

巻号頁・発行日

2021-04-01

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近年若者を中心に急速に広がりをみせている大麻濃縮物は、従来の大麻よりもΔ9-テトラヒドロカンナビノール(Δ9-THC)の含有量が多く、さらにΔ8-THCの含有も確認されている。これらの含有量を明らかにすることは、今後予想される様々な人体への影響、違法な流通ルートを把握するため重要となる。従来のクロマトグラフによる定量では、純度既知の標準品や検量線が必要なため操作が煩雑であったが、本研究では、標準品や検量線の必要がなく純度分析に適した核磁気共鳴装置を大麻濃縮物中のΔ9-THC及びΔ8-THCの定量に適用し、迅速かつ正確な定量法の開発を目指す。

著者

山室 匡史 宮本 重彦 立入 直紀 石井 歩 松田 駿太朗 岩田 祐子 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.786, (Released:2020-09-14)

参考文献数

31

被引用文献数

1

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The forensic identification of cannabis is performed by a combination of chemical analysis and morphological examination. Recently, molecular biological analysis using cannabis DNA information has been noticed as a new approach. In this study, the cannabis DNA detection kit using a DNA chromatography chip was developed, and the demonstration evaluation in the forensic chemical laboratory was carried out. The DNA detection kit of a “four-line version” which had the function to distinguish fiber-type from drug-type cannabis showed as high accuracy (98.3%) as the current identification method on cannabis identification. However, there was a tendency to mistake a part of the drug-type samples as “fiber-type cannabis”. In the kit of a “three-line version” which was specialized for the cannabis DNA detection, the accuracy of 99.0% was confirmed on the cannabis identification. There were no false positives throughout all evaluations. In addition, some of the combustion residues that could not be identified as cannabis by the current identification method were classified to be “cannabis positive” by the DNA detection kit, indicating the effectiveness of a new approach. As a result of this study, it was shown that the quick and accurate cannabis DNA analysis could be carried out by the DNA detection kit even by analytical chemists who didn't have expertise in molecular biology.