著者

辻川 健治 岡田 侑己 瀬川 尋貴 山室 匡史 桑山 健次 金森 達之 岩田 祐子 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.26, no.1, pp.103-113, 2021 (Released:2021-01-31)

参考文献数

13

被引用文献数

1

---

So-called “Ecstasy tablets” are tablets which are expected to contain 3,4-methylenedioxymethamphetamine (MDMA). However, they often contain different drugs such as methamphetamine (MA) and 4-bromo-2,5-dimethoxyphenethylamine (2C-B). A combination of Simon's reagent and Marquis reagent has been used as a field test for Ecstasy tablets in Japan. We examined the test performance for Ecstasy tablets. Mixtures of MDMA hydrochloride and lactose (1 or 5 mg), mixtures of MDMA hydrochloride and cellulose (1 mg) as well as pulverized Ecstasy tablets (1 mg) [main drugs: MDMA (n=39, 8.4 to 79.9% as MDMA hydrochloride), MA (n=9, 0.1 to 59.0% as MA hydrochloride), and 2C-B (n=6, 4.5 to 13.0% as 2C-B hydrochloride)] were placed on a white spot plate; then, the reagents were dropped. The color change was recorded by a digital camera. When 1 mg of the mixture of MDMA hydrochloride and the diluent was used, the lowest MDMA hydrochloride concentration giving positive was 25%, except when the combination of Simon's reagent and the mixture of MDMA hydrochloride and cellulose (1%) was used. When increasing the sample amount to 5 mg, enhancement of coloration for Marquis reagent was weaker than that for Simon's reagent because of the low sample solubility. All MDMA tablets was positive to Simon's reagent; however, 5 MDMA tablets, whose MDMA concentration was low (≤16.5% as MDMA hydrochloride), was negative for the Marquis reagent. Only 1 MA tablet (59% as MA hydrochloride) was positive for both tests. All 2C-B tablets were judged as negative for the Marquis reagent because of faint color change and influence of tablet color. We concluded that a combination of both reagents i) had acceptable sensitivity for MDMA tablets but may give some false negative results and ii) had insufficient sensitivity for MA tablets and 2C-B tablets. This study will provide useful information about the field test for Ecstasy tablets.

著者

山室 匡史 岡田 侑己 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 岩田 祐子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.27, no.1, pp.73-83, 2022 (Released:2022-01-31)

参考文献数

11

被引用文献数

1

---

In Japan, possession of germinable cannabis seeds for cultivation purposes is subject to prosecution. Cannabis seeds are marketed after being treated to prevent germination (heated or crushed). Currently, forensic examination of cannabis seeds is conducted by cultivating the seeds after germination tests for several weeks and then performing morphological observation and chemical analysis on the true leaves. In this study, we attempted to construct a rapid and simple method for the identification of cannabis seeds by combining the color reaction using 2,3,5-Triphenyl-2H-tetrazolium Chloride (TTC), a reagent that discriminates between living and dead cells, and DNA testing using a commercially available simple kit. The color reaction using TTC can determine the viability of peeled embryos within 20 min at 45 ℃ as previously reported. This method is effective for quickly determining whether a seed has been heat-treated or not. However, in the color reaction, a commercial health food seed that claimed to be unheated showed some coloration. This sample had been crushed to prevent germination and was easily identified as non-germinable by morphological examination. After the color reaction, the embryos could be directly used for DNA extraction without washing, and the DNA testing could be carried out in about 2 hours by following the instruction manual of the kit. By following the above procedure, it was possible to identify in one day whether a seed was a germinable cannabis or not, without the need to cultivate the plant. This method is expected to make a significant contribution to improving the efficiency of cannabis seed analysis.

著者

太田 彦人 櫻田 宏一 山室 匡史

出版者

科学警察研究所

雑誌

基盤研究(C)

巻号頁・発行日

2013-04-01

---

地下鉄サリン事件にて,救命時解毒剤2-PAMが投与されたにも関わらず亡くなられた被害者の解剖では,末梢AChE活性は回復したが脳内AChE活性は回復しなかった.これは2-PAMのBBB通過能が乏しいたためと考えられ,よりBBB通過能の高い解毒剤の開発を検討した.脂溶性オキシム4-PAOが十分なラットBBB通過能とAChE回復能を示すことがわかり,ラットを用いたin vivo解毒実験を行った.脳内AChE活性を12.5%まで阻害したラットに4-PAOを継続静注したところ,脳内AChE活性が定量的に回復,8mg/kg投与時で79.3%まで回復し,4-PAOが新たな解毒剤となり得る可能性が示された.

著者

岡田 侑己 瀬川 尋貴 山室 匡史 桑山 健次 辻川 健治 金森 達之 岩田 祐子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.28, no.1, pp.51-58, 2023 (Released:2023-01-31)

参考文献数

9

被引用文献数

1

---

We examined the decomposition behavior of 1-acyl-substituted derivatives of d-lysergic acid diethylamide (LSD), such as 1-cyclopropanecarbonyl LSD (1cP-LSD), 1-acetyl LSD (ALD-52), and 1-propionyl LSD (1P-LSD) through gas chromatography/mass spectrometry (GC/MS). It was reported previously that in methanol, 1cP-LSD completely decomposed into LSD during GC/MS. We found that in methanol, 1cP-LSD remained undecomposed during GC/MS, and the extent of decomposition varied based on the analyses performed in this study. As a result of a detailed examination, we deduced that the decomposition occurs at the inlet, regardless of the inlet temperature or the type of the inlet liner. We observed that the peak areas of 1cP-LSD decreased with the deterioration of the inlet liner, and this was considered to be a cause for the variation between different analyses. While the acetonitrile solution of 1cP-LSD provided relatively robust results, the other examined solvents showed a significant decomposition of 1cP-LSD and/or a sequent decrease in the peak area of 1cP-LSD with time after the replacement of the inlet liner. ALD-52 or 1P-LSD in acetonitrile were stable during GC/MS, however, they were unstable when methanol was used as a solvent, similar to 1cP-LSD. This suggested that a similar decomposition and/or a sequent decrease in the peaks of ALD-52 and 1P-LSD during GC/MS can be expected.

著者

山室 匡史 岡田 侑己 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 岩田 祐子

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.837, (Released:2022-06-16)

参考文献数

10

---

In Japan, forensic examination of cannabis seeds requires proof of “being cannabis” and “being germinable”, and is currently conducted by continuing cultivation of the seeds for several weeks after germination tests and performing morphological observations and chemical analysis on the true leaves. We have previously constructed a rapid and simple method for identification of cannabis seeds by combining the color reaction with DNA testing. In this study, the effectiveness of the cannabis seed identification methods by combining the color reaction, germination test, and DNA testing that does not involve a cultivation process was verified on seized cannabis seeds. As previously reported, the combination of the color reaction using 2,3,5-Triphenyl-2H-tetrazolium Chloride and DNA testing using a commercial kit proved that the seized materials were germinable cannabis seeds within one day. Furthermore, the germination test, in which the young roots and cotyledons were visually checked one week after sowing, was able to more directly confirm germination ability. In addition, DNA testing was possible for the young roots after germination, indicating that they were cannabis. This study shows that the methods of cannabis seed identification, which does not involve a cultivation process, are effective even for seized materials whose storage conditions are unknown.

著者

山室 匡史 宮本 重彦 立入 直紀 石井 歩 松田 駿太朗 岩田 祐子 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.26, no.1, pp.29-48, 2021 (Released:2021-01-31)

参考文献数

31

被引用文献数

3 1

---

The forensic identification of cannabis is performed by a combination of chemical analysis and morphological examination. Recently, molecular biological analysis using cannabis DNA information has been noticed as a new approach. In this study, the cannabis DNA detection kit using a DNA chromatography chip was developed, and the demonstration evaluation in the forensic chemical laboratory was carried out. The DNA detection kit of a “four-line version” which had the function to distinguish fiber-type from drug-type cannabis showed as high accuracy (98.3%) as the current identification method on cannabis identification. However, there was a tendency to mistake a part of the drug-type samples as “fiber-type cannabis”. In the kit of a “three-line version” which was specialized for the cannabis DNA detection, the accuracy of 99.0% was confirmed on the cannabis identification. There were no false positives throughout all evaluations. In addition, some of the combustion residues that could not be identified as cannabis by the current identification method were classified to be “cannabis positive” by the DNA detection kit, indicating the effectiveness of a new approach. As a result of this study, it was shown that the quick and accurate cannabis DNA analysis could be carried out by the DNA detection kit even by analytical chemists who didn't have expertise in molecular biology.

著者

山室 匡史 宮本 重彦 立入 直紀 石井 歩 松田 駿太朗 岩田 祐子 瀬川 尋貴 桑山 健次 辻川 健治 金森 達之 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

pp.786, (Released:2020-09-14)

参考文献数

31

被引用文献数

1

---

The forensic identification of cannabis is performed by a combination of chemical analysis and morphological examination. Recently, molecular biological analysis using cannabis DNA information has been noticed as a new approach. In this study, the cannabis DNA detection kit using a DNA chromatography chip was developed, and the demonstration evaluation in the forensic chemical laboratory was carried out. The DNA detection kit of a “four-line version” which had the function to distinguish fiber-type from drug-type cannabis showed as high accuracy (98.3%) as the current identification method on cannabis identification. However, there was a tendency to mistake a part of the drug-type samples as “fiber-type cannabis”. In the kit of a “three-line version” which was specialized for the cannabis DNA detection, the accuracy of 99.0% was confirmed on the cannabis identification. There were no false positives throughout all evaluations. In addition, some of the combustion residues that could not be identified as cannabis by the current identification method were classified to be “cannabis positive” by the DNA detection kit, indicating the effectiveness of a new approach. As a result of this study, it was shown that the quick and accurate cannabis DNA analysis could be carried out by the DNA detection kit even by analytical chemists who didn't have expertise in molecular biology.

著者

金森 達之 岩田 祐子 瀬川 尋貴 山室 匡史 桑山 健次 辻川 健治 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.24, no.2, pp.123-133, 2019 (Released:2019-07-31)

参考文献数

13

---

The isomers of fluoro-butyrylfentanyl, fluoro-isobutyrylfentanyl, and fluoro-methoxyacetylfentanyl, in which the position of fluorine on the N-phenyl ring varies, were synthesized, characterized, and differentiated by infrared (IR) spectroscopy, liquid chromatography/mass spectrometry (LC/MS), and gas chromatography/mass spectrometry (GC/MS). The isomers could be clearly differentiated by their IR spectra. In the LC/MS chromatograms, the separation of the fluoro-butyrylfentanyl and fluoro-isobutyrylfentanyl isomers was insufficient. However, in the GC/MS extracted ion chromatograms, all compounds were completely separated. The LC/MS and GC/MS mass spectra of the isomers were similar, demonstrating that it is difficult to distinguish the positional isomers of fluorinated fentanyl analogs by their mass spectra.

著者

金森 達之 岩田 祐子 辻川 健治 桑山 健次 山室 匡史 瀬川 尋貴 井上 博之

出版者

日本法科学技術学会

雑誌

日本法科学技術学会誌 (ISSN:18801323)

巻号頁・発行日

vol.21, no.2, pp.139-147, 2016 (Released:2016-07-23)

参考文献数

16

被引用文献数

1 3

---

Simultaneous analytical methods for 18 compounds of fentanyl and its analogues by thin-layer chromatography (TLC), gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) were developed. In TLC, fentanyl analogues were well separated by using toluene-acetone-28% aq. ammonia (20:10:0.3, by vol.) as a developing solvent. In GC/MS, fentanyl analogues, except for fentanyl and acetyl-α-methylfentanyl, could be separated on the extracted ion chromatograms (EIC) of the characteristic fragment ions of each compound. In LC/MS, fentanyl analogues could be separated on the EICs of the protonated molecule of each compound. All of the fentanyl analogues tested were identified correctly by using the combination of TLC, GC/MS and LC/MS.