著者

田島 研一 高橋 恒人 絵面 良男 木村 喬久

出版者

日本水産學會

雑誌

日本水産学会誌 (ISSN:00215392)

巻号頁・発行日

vol.50, no.1, pp.145-150, 1984

被引用文献数

1 16

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In a previous paper, we examined the toxicity of the extracellular protease of <i>A. salmonicida</i>, Ar-4 (EFDL) on yamabe (<i>Oncorhynchus masou f. ishikawai</i>) and goldfish (<i>Carassius auratus</i>). From these results, we considered the protease secreted by this bacterium was a causative agent of furunculosis.<br> In this paper, we observed enzymatic properties of the purified protease. The results obtained were summarized as follows:<br> 1. The molecular weight of purified protease was estimated to be 71, 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis.<br> 2. The purified protease showed maximal activity at pH 9.4 and 50°C. It was stable over the pH range 5.0 to 10.0 and was completely inactivated by temperature at 56°C.<br> 3. The protease was presumably classified an alkaline serine protease and it showed chymotryptic properties since it was significantly inhibited by diisopropylfluorophosphate (DFP) and tosyl-phenylalanine-chloromethyl ketone (TPCK) and hydrolyzed <i>N</i>-benzoyl-L-tyrosine ethyl ester (BTEE).

著者

吉水 守 絵面 良男

出版者

Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

雑誌

Microbes and Environments (ISSN:13426311)

巻号頁・発行日

vol.14, no.4, pp.269-275, 1999-12-31 (Released:2009-10-05)

参考文献数

25

被引用文献数

7 10

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Many bacteria producing anti-viral substances were isolated from the aquatic environment. Fish intestinal bacteria such as Aeromonas spp. and Vibrio spp. producing anti-viral substances were isolated from intestinal contents of masu salmon (Oncorhynchus masou), Japanese flounder (Paralichthys olivaceus) and barfin flounder (Verasper moseri). These Aeromonas strains produced anti-infectious hematopoietic necrosis virus (IHNV) substances and Vibrio strains showed anti-IHNV, Oncorhynchus masou virus (OMV) and barfin flounder nervous necrosis virus (BF-NNV) activities. When Aeromonas spp. strains M-26 and M-38 were mixed with food pellets and fed to rainbow trout (O. mykiss) and masu salmon, both bacteria became dominant in the intestinal microflora and anti-IHNV activity was observed in homogenates of intestinal contents. These rainbow trout and masu salmon fed the Aeromonas spp. showed more resistance to the artificial IHNV challenge test. Barfin flounder fed Vibrio sp. strain 2IF6a with Altemia salina showed the anti-OMV and BF-NNV activities in the intestinal contents. Larvae fed the Vibrio sp. showed a higher survival rate than the fish cultured using the virus free sea water.

著者

田島 研一 高橋 恒人 絵面 良男 木村 喬久

出版者

北海道大学水産学部 = FACULTY OF FISHERIES, HOKKAIDO UNIVERSITY

雑誌

北海道大學水産學部研究彙報 (ISSN:00183458)

巻号頁・発行日

vol.34, no.2, pp.111-123, 1983-06

著者

田島 研一 高橋 恒人 絵面 良男 木村 喬久

出版者

北海道大学水産学部 = FACULTY OF FISHERIES, HOKKAIDO UNIVERSITY

雑誌

北海道大學水産學部研究彙報 (ISSN:00183458)

巻号頁・発行日

vol.34, no.2, pp.104-110, 1983-06