著者

蒔苗 靖子 渡部 徹 大村 達夫 遠藤 銀朗

出版者

日本水環境学会

雑誌

水環境学会誌 = Journal of Japan Society on Water Environment (ISSN:09168958)

巻号頁・発行日

vol.23, no.12, pp.771-777, 2000-12-10

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Outbreaks of infectious diseases by new pathogenic microorganisms through water utilization have been reported in recent years. Especially, Legionnaires' disease has been recognized as a serious problem in modern societies since a high concentration of <i>Legionella pneumophila</i> often exist in water environment (such as cooling tower, bath water, recreational water) close to human activities. In this study, the quantitative detection method (colony-hybridization) for <i>L. pneumophila</i> was developed by combining the hybridization with the plate culture method. The developed detection method enabled <i>L. pneumophila</i> to be enumerated in various water samples. It was possible to quantify <i>L. pneumophila</i> without interferences of other bacteria by this method in case that the number of total coliforms on the plate was less than 10³ CFU.