- 著者
- 鈴木 久美子 采 輝昭 山崎 幹夫
- 出版者
- 公益社団法人日本薬学会
- 雑誌
- 藥學雜誌 (ISSN:00316903)
- 巻号頁・発行日
- vol.108, no.3, pp.221-225, 1988
- 被引用文献数
- 7
A fraction causing diarrhea to mice with a hemolytic activity was previously isolated from the aqueous extract of a poisonous mushroom Rhodophyllus rhodopolius. In order to clarify the relationship between the enterotoxicity and hemolytic activity, the hemolysin was purified. The hemolysin which was partially purified by DEAE-cellulose column chromatography, ammonium sulfate precipitation, and Sephacryl S-300 column chromatography, caused diarrhea and intestinal hemorrhage to suckling mice. The molecular weight of the hemolysin was estimated to be about 40000 by SDS-slab polyacrylamide gel electrophoresis and gel filtration. The hemolytic activity of the hemolysin was observed to be relatively heat-stable in the crude extract and dependent on the temperature and concentration of erythrocytes.
1 0 0 0 OA クサウラベニタケの毒性成分の研究(第2報) : 溶血素の精製と溶血条件の検討
- 著者
- 鈴木 久美子 采 輝昭 山崎 幹夫
- 出版者
- 公益社団法人 日本薬学会
- 雑誌
- YAKUGAKU ZASSHI (ISSN:00316903)
- 巻号頁・発行日
- vol.108, no.3, pp.221-225, 1988-03-25 (Released:2008-05-30)
- 参考文献数
- 12
- 被引用文献数
- 1 7
A fraction causing diarrhea to mice with a hemolytic activity was previously isolated from the aqueous extract of a poisonous mushroom Rhodophyllus rhodopolius. In order to clarify the relationship between the enterotoxicity and hemolytic activity, the hemolysin was purified. The hemolysin which was partially purified by DEAE-cellulose column chromatography, ammonium sulfate precipitation, and Sephacryl S-300 column chromatography, caused diarrhea and intestinal hemorrhage to suckling mice. The molecular weight of the hemolysin was estimated to be about 40000 by SDS-slab polyacrylamide gel electrophoresis and gel filtration. The hemolytic activity of the hemolysin was observed to be relatively heat-stable in the crude extract and dependent on the temperature and concentration of erythrocytes.