著者
高島 大典
出版者
栃木県農業試験場
巻号頁・発行日
no.28, pp.47-54, 1982 (Released:2011-03-05)
著者
世取山 守 伊藤 功 高島 大典 正山 征洋 西岡 五夫
出版者
公益社団法人日本薬学会
雑誌
藥學雜誌 (ISSN:00316903)
巻号頁・発行日
vol.100, no.6, pp.611-614, 1980-06-25

Cannabinoids (CN) of CBDA stock, Tochishi No. 1 stock and crossed stock were determined on a high-pressure liquid chromatograph equipped with a LiChrosorb RP-18 column (25 cm×4 mm) by elution with a mixture of methanol and 0.02 N sulphuric acid (4 : 1). The CBDA stock was found to contain cannabidiolic acid (CBDA) and cannabichromenic acid (CBCA). Tochishi No. 1 stock was detected to have thtrahydrocannabinolic acid (THCA), CBCA and a small amount of CBDA. F_1 was obtained by crossing CBDA stock (female) and Tochishi No. 1 stock (male). All 79 F_1 specimens were found to contain CBDA, THCA and CBCA. Therefore F_1 was designated the median type. F_2 was then obtained by crossing F_1 and out of 199 F_2 specimens, 58,40 and 101 stocks were designated as CBDA stock type, Tochishi No. 1 stock type and the median type by CN analysis, respectively. F_3 was also gained by crossing CBDA stock type of F_2 and all 166 F_3 specimens were CBDA stock type. F_4,which was obtained by crossing F_3,was also CBDA stock type. In addition, the fibers of F_4 were found to be better than those of the original CBDA stock, and F_4 contained a negligible amount of THCA.