著者
Atsushi Ishihara Kana Ando Anna Yoshioka Koichi Murata Yu Kokubo Noriko Morimoto Naoki Ube Yukinori Yabuta Makoto Ueno Shin-ichi Tebayashi Kotomi Ueno Kumiko Osaki-Oka
出版者
Pesticide Science Society of Japan
雑誌
Journal of Pesticide Science (ISSN:1348589X)
巻号頁・発行日
vol.44, no.2, pp.89-96, 2019-05-20 (Released:2019-05-20)
参考文献数
36
被引用文献数
3

We investigated the effect of treatment with hot water extracts from the spent mushroom substrates (SMSs) of Lentinula edodes and Hypsizygus marmoreus on the resistance of rice leaves to Pyricularia oryzae infection. The spraying of the SMS extracts clearly suppressed the development of lesions caused by Py. oryzae infection. The accumulation of phytoalexins momilactones A and B, oryzalexin A, and sakuranetin was markedly induced by the spraying of extracts. The enhanced expression of defense related genes PR1b and PBZ was also found in leaves sprayed with the extracts. Treatments with the extracts also affected phytohormone levels. The levels of N6-(Δ2-isopentenyl)adenine and trans-zeatin markedly increased in response to treatment, whereas the levels of salicylic and jasmonic acids were largely unchanged.
著者
Atsushi Ishihara Kana Ando Anna Yoshioka Koichi Murata Yu Kokubo Noriko Morimoto Naoki Ube Yukinori Yabuta Makoto Ueno Shin-ichi Tebayashi Kotomi Ueno Kumiko Osaki-Oka
出版者
Pesticide Science Society of Japan
雑誌
Journal of Pesticide Science (ISSN:1348589X)
巻号頁・発行日
pp.D18-063, (Released:2019-03-12)
参考文献数
36
被引用文献数
3

We investigated the effect of treatment with hot water extracts from the spent mushroom substrates (SMSs) of Lentinula edodes and Hypsizygus marmoreus on the resistance of rice leaves to Pyricularia oryzae infection. The spraying of the SMS extracts clearly suppressed the development of lesions caused by Py. oryzae infection. The accumulation of phytoalexins momilactones A and B, oryzalexin A, and sakuranetin was markedly induced by the spraying of extracts. The enhanced expression of defense related genes PR1b and PBZ was also found in leaves sprayed with the extracts. Treatments with the extracts also affected phytohormone levels. The levels of N6-(Δ2-isopentenyl)adenine and trans-zeatin markedly increased in response to treatment, whereas the levels of salicylic and jasmonic acids were largely unchanged.
著者
Tomoyasu Taguchi Atsushi Ishihara Hiromitsu Nakajima
出版者
日本マイコトキシン学会
雑誌
マイコトキシン (ISSN:02851466)
巻号頁・発行日
vol.65, no.2, pp.131-142, 2015-07-31 (Released:2015-09-01)
参考文献数
37

Fungal contamination of food is one of the most important food safety concerns, however effective techniques to prevent fungal contamination and/or control fungal growth in foods have not yet been established. Some endogenous volatile compounds in plants have antibacterial and/or antifungal activities. For example, when some plants are damaged, they release aliphatic aldehydes with six or nine carbons to protect themselves from microbial infection. Patulin is the major mycotoxin that contaminates apples and apple juice worldwide, and it is regulated in many countries. Patulin has been shown to be immunotoxic and neurotoxic by animal experiments. Many species of Penicillium and Aspergillus produce patulin, but P. expansum is the most typical species responsible for patulin contamination in apples. We attempted to assess the effects of aliphatic aldehydes on P. expansum. Aliphatic aldehydes composed of 3–6 carbons bearing an E double bond at the α-position completely inhibited the fungal growth and suppressed colony formation from spores at relatively low concentrations. On the basis of the structure-activity relationship, the antifungal activity of the compounds is probably attributable to the interaction of the aldehyde group with biological macromolecules. On the other hand, aliphatic aldehydes with 8–10 carbons stimulated patulin production by P. expansum. The results of a reverse transcription-quantitative polymerase chain reaction analysis suggested that the stimulation was partially due to enhanced transcription of some patulin biosynthetic genes. The effects of volatiles of apple on patulin production by P. expansum were also studied. Some volatile compounds, that is, 2-mehtylbutanoic acid and ethyl 2-methylbutanoate, were found to stimulate patulin production. These findings will contribute to the development of new techniques to prevent and control fungal and mycotoxin contamination of foods.