著者

Cheng Yang Jun Teng Chao Ning Wenwen Wang Shuai Liu Qin Zhang Dan Wang Hui Tang

出版者

Japan Poultry Science Association

雑誌

The Journal of Poultry Science (ISSN:13467395)

巻号頁・発行日

pp.0210138, (Released:2022-05-25)

被引用文献数

2

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Body measurement traits (BMTs), which are classical quantitative traits of vital responses to body growth, have been studied in pigs, cattle, and sheep for several decades. In chickens, BMTs mainly cover body slope length, keel length, chest width, chest depth, tibia length, and tibia diameter; however, their genetic markers are yet to be considered. In this study, the Wenshang Barred chicken, a meat-egg-type native breed in China, was used to investigate the association between BMTs and the expression of growth-related genes, including GH, IGF1, IGF2, GHRL, IGF1R, IGFBP2, GHF-1, and TSHB. The results revealed that the single nucleotide polymorphism (SNP) rs3138025 in GH was significantly associated with keel length (P = 0.0455 < 0.05), rs313810945 in IGF2 was significantly correlated with chest width (P = 0.0454 < 0.05) and chest depth (P = 0.0259 < 0.05), and rs317298536 in TSHB significantly affected chest depth (P = 0.0399 < 0.05). The SNPs were associated with traits reflecting body size and were potentially involved in bone growth, which was consistent with studies in humans, rodents, and other vertebrate species. In addition, a borderline significant association was found between rs317298536 and body weight (P = 0.0604). These polymorphic sites may be treated as candidate genetic markers in breeding programs involving Wenshang Barred chickens.

著者

Govindaraj USHA Ramesh PRAKASH Karuppasamy KARPAGALAKSHMI Sundaram RAMALAKSHMI Lakshminarayanan PIRAMUTHU Cheng YANG Narayanan SELVAPALAM

出版者

The Japan Society for Analytical Chemistry

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.36, no.11, pp.1365-1369, 2020-11-10 (Released:2020-11-10)

参考文献数

18

被引用文献数

3

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An acriflavine-graphene oxide (GAF) supramolecular assembly has been prepared from water-soluble graphene oxide (GO) and a fluorescent dye, acriflavine (AF). Upon binding this non-covalently to the GO, the fluorescence of acriflavine has been “turned off” effectively, competitive binding potential of the sensor substrates such as ATP, ADP, AMP and the pyrophosphate weakens the supramolecular assembly of GAF, which allows the release of acriflavine quantitatively, which also “turns-on” the fluorescence of the dye under UV irradiation. Interestingly, GAF displayed the highest sensitivity towards ATP within the family of adenosine phosphates. We have developed a naked eye detection method for the adenosine phosphates biomolecules. For the first time, acriflavine has been utilized for the sensing of adenosine phosphates in combination with GO, which can be useful for the detection of other biomolecules.

著者

Yun-Ru CHEN David TSENG Chieh TING Pei-Shou HSU Tsu-Hsien WU Siling ZHONG En-Cheng YANG

出版者

日本毒性学会

雑誌

日本毒性学会学術年会

巻号頁・発行日

vol.46, pp.S7-1, 2019

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<p>Multiple factors have been associated with the honey bee colony losses in the past 25 years. Accumulating evidence indicates that at sublethal doses, neonicotinoids cause honey bee brain dysfunction and reduce immunocompetence, leading to impaired navigation and olfactory learning and memory, and susceptibility to pathogens. Moreover, such pesticides disturb the reproductive system of queen bees, i.e., the number of eggs and viability of sperm stored in the spermatheca of queen bees, thereby reducing the numbers of adult bees and broods. Pesticide exposure during the larval development stages prolongs larval development and shortens adult longevity. In addition, the density of synaptic units in the calyces of mushroom bodies in the heads decreases; this effect has been further associated with the abnormal olfactory learning ability of adult honey bees exposed to sublethal doses of imidacloprid during the larval stage. Therefore, numerous physiological aspects of honey bees might be altered after exposure to pesticides at sublethal doses, regardless of the developmental stage. We evaluated the impact of the most widely used neonicotinoids—imidacloprid—on different developmental stages of honey bees by profiling the transcriptomes of worker bees with imidacloprid during only the larval stage. Our results confirm that even intaking 1 ppb imidacloprid during only the larval stage would be enough to severely impact a bee's gene expression. The existence of many differentially expressed genes may reflect or result in honey bee disorder.</p>