- 著者
- Vivian Bee Chin LEE Noor Faizah MOHD-NAIM Eiichi TAMIYA Minhaz Uddin AHMED
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.34, no.1, pp.7-18, 2018-01-10 (Released:2018-01-10)
- 参考文献数
- 85
- 被引用文献数
- 1 76
Electrochemical bio-sensing using paper-based detection systems is the main focus of this review. The different existing designs of 2-dimensional and 3-dimensional sensors, and fabrication techniques are discussed. This review highlights the effect of adopting different sensor designs, distinct fabrication techniques, as well as different modification methods, in order to produce reliable and reproducible reading. The use of various nanomaterials have been demonstrated in order to modify the surface of electrodes during fabrication to further enhance the signal for subsequent analysis. The reviewed sensors were classified into categories based on their applications, such as diagnostics, environmental and food testing. One of the major advantages of using paper-based electrochemical sensors is the potential for miniaturization, which only requires relatively small amount of samples, and the low cost for the purpose of mass production. Additionally, most of the devices reviewed were made to be portable, making them well-suited for on-site detection. Finally, paper-based detection is an ideal platform to fabricate cost-effective, user-friendly and sensitive electrochemical biosensors, with large capacity for customization depending on functional needs.
- 著者
- 大野 信子 岡留 美穂 李 晶 オオノ ノブコ オカドメ ミホ / Nobuko OHNO Miho OKADOME Chin LEE
- 雑誌
- 和洋女子大学紀要. 家政系編
- 巻号頁・発行日
- vol.43, pp.203-212, 2003-03-31
リンゴ果実青カビ病菌Penicillium expansumの酵素の生産とペクチン分解酵素を精製し、酵素化学的性質を調べた。供試菌株は、ペクチンー無機塩類培地で、比較的短時間に、培養濾液の中に、ポリガラクツロナーゼを生産した。本菌株を窒素源としてリン酸アンモニウム(0.5%)、ペクチン(2.0%)含む無機塩類培地を用いて30℃において、4日間振とう培養した場合、培養液中の総ポリガラクツロナーゼの活性が最大(1.56U/ml)に達した。培養濾液中からDEAE-セルロースクロマトグラフィーで2つの活性画分(ポリガラクツロナーゼI、II)を精製した。それぞれポリガラクツロナーゼIとIIの活性の最適pHは4.8と5.5、最適温度は同じく40℃であった。両酵素とも0~40℃、pH3~7.5の範囲で安定であった。両酵素の活性は1mM Ca^<2+>、1mM Mg^<2+>によってそれぞれ約50~60%と約70~80%までに阻害された。